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Titolo:
ACTIVATION OF AMP-DEAMINASE IN HUMAN ERYTHROCYTES BY CALCIUM-IONS
Autore:
ENGSTROM I; WALDENSTROM A; RONQUIST G;
Indirizzi:
UNIV UPPSALA HOSP,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN NORRLAND UNIV HOSP,DEPT INTERNAL MED UMEA SWEDEN
Titolo Testata:
Scandinavian journal of clinical & laboratory investigation
fascicolo: 4, volume: 56, anno: 1996,
pagine: 345 - 350
SICI:
0036-5513(1996)56:4<345:AOAIHE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURINE NUCLEOTIDE CYCLE; HUMAN RED-CELL; DEFICIENCY; PURIFICATION; DEGRADATION; METABOLISM; ENZYME;
Keywords:
AMP DEAMINASE; CALCIUM IONS; ENERGY CHARGE; ERYTHROCYTES; IMP; IONOPHORE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
I. Engstrom et al., "ACTIVATION OF AMP-DEAMINASE IN HUMAN ERYTHROCYTES BY CALCIUM-IONS", Scandinavian journal of clinical & laboratory investigation, 56(4), 1996, pp. 345-350

Abstract

We examined 5'-AMP aminohydrolase (AMP deaminase, EC 3.5.4.6. AMPD) together with metabolite changes in intact red blood cells from normal human adults under different incubation conditions. Moderate changes occurred in erythrocyte adenylates upon incubation for 1 h at 37 degrees C in the absence of glucose and the concentration of IMP was negligible. Calcium in the incubation medium, at concentrations up to 1.50 mmol l(-1) did not influence the nucleotide pattern. Inclusion of the ionophore A 23187 in the incubation medium resulted in a sharp decrease of ATP content at 60 min and increases in AMP and ADP concentrations. A maximally low level of ATP was observed at 60 min of incubation in the presence of both the ionophore and 0.25 mmol l(-1) of external calcium. With increasing concentrations of calcium up to 1.50 mmol l(-1) in the presence of the ionophore, a significant rise occurred in both IMP and ATP concentrations, whether glucose was present or not, and significant correlations existed between the concentrations of calcium and IMP. The presence of the ionophore together with glucose but withoutextra calcium in the incubation medium produced the highest concentrations of lactate, indicative of an enhanced glycolytic flux under these conditions. The stimulatory effect of intracellular calcium ions on AMPD activity is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 17:01:29