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Titolo:
MODIFICATION OF DOPAMINE TRANSPORTER FUNCTION - EFFECT OF REACTIVE OXYGEN SPECIES AND DOPAMINE
Autore:
BERMAN SB; ZIGMOND MJ; HASTINGS TG;
Indirizzi:
UNIV PITTSBURGH,DEPT NEUROL,568 CRAWFORD HALL PITTSBURGH PA 15260 UNIV PITTSBURGH,DEPT NEUROL PITTSBURGH PA 15260 UNIV PITTSBURGH,DEPT NEUROSCI PITTSBURGH PA 15260
Titolo Testata:
Journal of neurochemistry
fascicolo: 2, volume: 67, anno: 1996,
pagine: 593 - 600
SICI:
0022-3042(1996)67:2<593:MODTF->2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC ACID; CENTRAL-NERVOUS-SYSTEM; H-3 DOPAMINE; LIPID-PEROXIDATION; NEUROTRANSMITTER TRANSPORTERS; ASCORBIC-ACID; STRIATAL SYNAPTOSOMES; OXIDATIVE PATHWAYS; PROTEIN OXIDATION; FREE-RADICALS;
Keywords:
FREE RADICALS; DOPAMINE UPTAKE; NEUROTOXICITY; QUINONES; PARKINSONS DISEASE; ASCORBATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
S.B. Berman et al., "MODIFICATION OF DOPAMINE TRANSPORTER FUNCTION - EFFECT OF REACTIVE OXYGEN SPECIES AND DOPAMINE", Journal of neurochemistry, 67(2), 1996, pp. 593-600

Abstract

Dopamine can oxidize to form reactive oxygen species and quinones, and we have previously shown that dopamine quinones bind covalently to cysteinyl residues on striatal proteins, The dopamine transporter is one of the proteins at risk for this modification, because it has a highaffinity for dopamine and contains several cysteinyl residues. Therefore, we tested whether dopamine transport in rat striatal synaptosomescould be affected by generators of reactive oxygen species, includingdopamine. Uptake of [H-3]dopamine (250 nM) was inhibited by ascorbate(0.85 mM; -44%), and this inhibition was prevented by the iron chelator diethylenetriaminepentaacetic acid (1 mM), suggesting that ascorbate was acting as a prooxidant in the presence of iron. Preincubation with xanthine (500 mu M) and xanthine oxidase (50 mU/ml) also reduced [H-3]dopamine uptake (-76%). Preincubation with dopamine (100 mu M) caused a 60% inhibition of subsequent [H-3]dopamine uptake. This dopamine-induced inhibition was attenuated by diethylenetriaminepentaacetic acid (1 mM), which can prevent iron-catalyzed oxidation of dopamine during the preincubation, but was unaffected by the monoamine oxidase inhibitor pargyline (10 mu M). None of these incubations caused a loss of membrane integrity as indicated by lactate dehydrogenase release. Thesefindings suggest that reactive oxygen species and possibly dopamine quinones can modify dopamine transport function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:16:28