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Titolo:
DEVELOPMENT OF A SIMPLE CHEMICALLY-DEFINED MEDIUM FOR PORPHYROMONAS-GINGIVALIS - REQUIREMENT FOR ALPHA-KETOGLUTARATE
Autore:
MILNER P; BATTEN JE; CURTIS MA;
Indirizzi:
ST BARTHOLOMEWS & ROYAL LONDON SCH MED & DENT,MRC,MOLEC PATHOGENESIS GRP,DEPT ORAL MICROBIOL LONDON E1 2AA ENGLAND ST BARTHOLOMEWS & ROYAL LONDON SCH MED & DENT,MRC,MOLEC PATHOGENESIS GRP,DEPT ORAL MICROBIOL LONDON E1 2AA ENGLAND
Titolo Testata:
FEMS microbiology letters
fascicolo: 2-3, volume: 140, anno: 1996,
pagine: 125 - 130
SICI:
0378-1097(1996)140:2-3<125:DOASCM>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; ENZYME-ACTIVITY; GROWTH; W50;
Keywords:
PORPHYROMONAS GINGIVALIS; PERIODONTAL DISEASE; CHEMICALLY DEFINED MEDIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
P. Milner et al., "DEVELOPMENT OF A SIMPLE CHEMICALLY-DEFINED MEDIUM FOR PORPHYROMONAS-GINGIVALIS - REQUIREMENT FOR ALPHA-KETOGLUTARATE", FEMS microbiology letters, 140(2-3), 1996, pp. 125-130

Abstract

The aim of this study was the development of a simple, defined mediumfor the growth of laboratory and clinical isolates of Porphyromonas gingivalis. A medium was designed in which the carbon and nitrogen requirements were provided by a single protein source - bovine serum albumin. High cell yields were achieved in this medium but growth was accompanied by a heavy blackening of the cells due to the deposition of metal sulfide(s), most probably iron(II) sulfide, at the cell surface. Good growth in the absence of blackening was achieved when the iron saltin the medium was substituted with alpha-ketoglutarate, The resultantalpha-ketoglutarate/BSA medium was able to support the growth of all laboratory and clinical P. gingivalis strains examined and should prove useful in the investigation of the physiology and nutritional regulation of virulence of this organism.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:23:15