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Titolo:
TRANSCRIPTIONAL REGULATION OF INOS BY IL-1-BETA IN CULTURED RAT PULMONARY-ARTERY SMOOTH MUSCLE-CELLS
Autore:
WONG HR; FINDER JD; WASSERLOOS K; LOWENSTEIN CJ; GELLER DA; BILLIAR TR; PITT BR; DAVIES P;
Indirizzi:
UNIV PITTSBURGH,SCH MED,DEPT PHARMACOL PITTSBURGH PA 15261 UNIV PITTSBURGH,SCH MED,DEPT PHARMACOL PITTSBURGH PA 15261 UNIV PITTSBURGH,SCH MED,DEPT ANESTHESIOL CRIT CARE MED,DIV PEDIAT CRIT CARE MED PITTSBURGH PA 15261 UNIV PITTSBURGH,SCH MED,DEPT PEDIAT PITTSBURGH PA 15261 UNIV PITTSBURGH,SCH MED,DEPT SURG PITTSBURGH PA 15261 JOHNS HOPKINS UNIV,SCH MED,DEPT MED BALTIMORE MD 21205
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 1, volume: 15, anno: 1996,
pagine: 166 - 171
SICI:
1040-0605(1996)15:1<166:TROIBI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; KAPPA-B ACTIVATION; INTERFERON-GAMMA; INDUCTION; GENE; LIPOPOLYSACCHARIDE; EXPRESSION; INTERLEUKIN; INHIBITORS; PROMOTER;
Keywords:
PULMONARY VASCULATURE; NUCLEAR FACTOR-KAPPA-B; ELECTROPHORETIC MOBILITY SHIFT ASSAY; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERLEUKIN-1-BETA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
H.R. Wong et al., "TRANSCRIPTIONAL REGULATION OF INOS BY IL-1-BETA IN CULTURED RAT PULMONARY-ARTERY SMOOTH MUSCLE-CELLS", American journal of physiology. Lung cellular and molecular physiology, 15(1), 1996, pp. 166-171

Abstract

Interleukin-1 beta (IL-1 beta) is the critical cytokine affecting peripheral vascular expression of inducible nitric oxide synthase (iNOS). Accordingly, we sought to determine a role for IL-1 beta in stimulating iNOS transcription in cultured rat pulmonary artery smooth muscle cells (RPASMC). Treatment of RPASMC with IL-1 beta caused a concentration-dependent increase in iNOS gene expression by Northern and Western blotting. To demonstrate IL-1 beta-mediated transcriptional activation, we used transient liposome-mediated transfection of RPASMC with promoter-luciferase constructs containing deletional mutations of the marine macrophage iNOS 5' flanking promoter region. IL-1 beta increased promoter activity approximately two- to threefold over baseline in fragments ranging from -1592 (full-length) to -242 bp. Activity was lost, however, when the promoter fragment was shorter than -242 bp. IL-1 beta-mediated increases in steady-state iNOS mRNA were sensitive to pyrrolidine dithiocarbamate (PDTC), an inhibitor of NF-kappa B activation. Nuclear proteins from IL-1 beta-stimulated cells demonstrated PDTC-sensitive binding to an oligonucleotide containing the sequence for the NF-kappa B binding element present in the region between -242 and -42 bp. These data document that IL-1 beta, by itself, increases iNOS expression in RPASMC by transcriptional activation, mediated in part by NF-kappa B.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:27:37