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Titolo:
ANAEROBIC METABOLISM OF 2-HYDROXYBENZOIC ACID (SALICYLIC-ACID) BY A DENITRIFYING BACTERIUM
Autore:
BONTING CFC; FUCHS G;
Indirizzi:
UNIV FREIBURG,INST BIOL 2,SCHANZLESTR 1 D-79104 FREIBURG GERMANY UNIV FREIBURG,INST BIOL 2 D-79104 FREIBURG GERMANY UNIV ULM,ANGEW MIKROBIOL ABT D-89069 ULM GERMANY
Titolo Testata:
Archives of microbiology
fascicolo: 6, volume: 165, anno: 1996,
pagine: 402 - 408
SICI:
0302-8933(1996)165:6<402:AMO2A(>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHODOPSEUDOMONAS-PALUSTRIS; ENZYMATIC REDUCTION; AROMATIC METABOLISM; PHENOLIC-COMPOUNDS; BENZOATE-COENZYME; KEY REACTION; DEGRADATION; LIGASE; COA; PURIFICATION;
Keywords:
SALICYLIC ACID; BENZOYL-COA; 2-HYDROXYBENZOYL-COA; 2-HYDROXYBENZOATE-COA LIGASE; 2-HYDROXYBENZOYL-COA REDUCTASE (DEHYDROXYLATING);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
C.F.C. Bonting e G. Fuchs, "ANAEROBIC METABOLISM OF 2-HYDROXYBENZOIC ACID (SALICYLIC-ACID) BY A DENITRIFYING BACTERIUM", Archives of microbiology, 165(6), 1996, pp. 402-408

Abstract

The anaerobic metabolism of 2-hydroxybenzoic acid (salicylic acid) was studied in a denitrifying bacterium. Cells grown with 2-hydroxybenzoate were simultaneously adapted to degrade benzoate. Extract of these cells formed benzoate or benzoyl-CoA when incubated under reducing conditions with salicylate, MgATP, and coenzyme A, suggesting a degradation of 2-hydroxybenzoate via benzoate or benzoyl-CoA. This suggestion was supported by enzyme activity measurements. In extracts of 2-hydroxybenzoate-grown cells, the following enzyme activities were detected: two CoA ligases, one specific for 2-hydroxybenzoate, the other for benzoate, and two different enzyme activities catalyzing the reductive transformation of 2-hydroxybenzoyl-CoA. These findings suggest a degradation of salicylic acid by two new enzymes, 2-hydroxybenzoate-CoA ligase(AMP-forming) and 2-hydroxybenzoyl-CoA reductase (dehydroxylating), catalyzing (1) 2-hydroxybenzoate + MgATP + CoASH --> 2-hydroxybenzoyl-CoA MgAMP + PPi (2) 2-hydroxybenzoyl-CoA + 2[H] --> benzoyl-CoA + H2O Benzoyl-CoA was dearomatized by reduction of the ring. This represents another case in which benzoyl-CoA is a central intermediate in anaerobic aromatic metabolism.

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Documento generato il 08/07/20 alle ore 07:32:43