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Titolo:
LEPTO DIPSTICK, A DIPSTICK ASSAY FOR DETECTION OF LEPTOSPIRA-SPECIFICIMMUNOGLOBULIN-M ANTIBODIES IN HUMAN SERA
Autore:
GUSSENHOVEN GC; VANDERHOORN MAWG; GORIS MGA; TERPSTRA WJ; HARTSKEERL RA; MOL BW; VANINGEN CW; SMITS HL;
Indirizzi:
ROYAL TROP INST,DEPT BIOMED RES,MEIBERGDREEF 39 NL-1105 AZ AMSTERDAM NETHERLANDS ROYAL TROP INST,DEPT BIOMED RES NL-1105 AZ AMSTERDAM NETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,LAB CLIN EPIDEMIOL NL-1105 AZ AMSTERDAM NETHERLANDS FDN ADVANCEMENT PUBL HLTH & ENVIRONM PROTECT BILTHOVEN NETHERLANDS
Titolo Testata:
Journal of clinical microbiology
fascicolo: 1, volume: 35, anno: 1997,
pagine: 92 - 97
SICI:
0095-1137(1997)35:1<92:LDADAF>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
G.C. Gussenhoven et al., "LEPTO DIPSTICK, A DIPSTICK ASSAY FOR DETECTION OF LEPTOSPIRA-SPECIFICIMMUNOGLOBULIN-M ANTIBODIES IN HUMAN SERA", Journal of clinical microbiology, 35(1), 1997, pp. 92-97

Abstract

We studied a dipstick assay for the detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human serum samples. A high degree of concordance was observed between the results of the dipstick assay and an IgM enzyme-linked immunosorbent assay (ELISA), Application ofthe dipstick assay for the detection of acute leptospirosis enabled the accurate identification, early in the disease, of a high proportionof the cases of leptospirosis. Analysis of a second serum sample is recommended, in order to determine seroconversion or increased stainingintensity. All serum samples from the patients who were confirmed to be positive for leptospirosis by either a positive microscopic agglutination test or a positive culture but were found to be negative by thedipstick assay were also judged to be negative by the IgM ELISA or revealed borderline titers by the IgM ELISA. Some cross-reactivity was observed for sera from patients with diseases other than leptospirosis,and this should be taken into account in the interpretation of test results. The dipstick assay is easy to perform, can be performed quickly, and requires no electricity or special equipment, and the assay components, a dipstick and a staining reagent, can be stored for a prolonged period without a loss of reactivity, even at elevated temperatures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 18:43:47