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Titolo:
SYNAPTIC-INTERACTIONS OF RETROGRADELY LABELED HYPOGLOSSAL MOTONEURONSWITH SUBSTANCE P-LIKE IMMUNOREACTIVE NERVE-TERMINALS IN THE CAT - A DUAL-LABELING ELECTRON-MICROSCOPIC STUDY
Autore:
GATTI PJ; COLEMAN WC; SHIRAHATA M; JOHNSON TA; MASSARI VJ;
Indirizzi:
HOWARD UNIV,COLL MED,DEPT PHARMACOL,520 W ST NW WASHINGTON DC 20059 JOHNS HOPKINS MED INST,DEPT ENVIRONM HLTH SCI,DIV PHYSIOL BALTIMORE MD 21205 HOWARD UNIV,COLL MED,DEPT VET SERV WASHINGTON DC 20059
Titolo Testata:
Experimental Brain Research
fascicolo: 2, volume: 110, anno: 1996,
pagine: 175 - 182
SICI:
0014-4819(1996)110:2<175:SORLHM>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTAINING AXON TERMINALS; BRAIN-STEM; HORSERADISH-PEROXIDASE; MOTO-NEURONS; RAT; NUCLEUS; SYSTEM; SEROTONIN; RECEPTORS; ARCHITECTURE;
Keywords:
HYPOGLOSSAL MOTONEURONS; RETROGRADE TRANSPORT; SYNAPTIC INTERACTIONS; SUBSTANCE P; CAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Gatti et al., "SYNAPTIC-INTERACTIONS OF RETROGRADELY LABELED HYPOGLOSSAL MOTONEURONSWITH SUBSTANCE P-LIKE IMMUNOREACTIVE NERVE-TERMINALS IN THE CAT - A DUAL-LABELING ELECTRON-MICROSCOPIC STUDY", Experimental Brain Research, 110(2), 1996, pp. 175-182

Abstract

This study has investigated the synaptic interactions between hypoglossal motoneurons and substance P (SP)-immunoreactive terminals. Cholera toxin B conjugated to horseradish peroxidase was injected into the tip of the tongue on the right side of six ketamine-anesthetized cats. Two to five days later, the animals were killed. Cells containing HRP were labeled with a histochemical reaction utilizing tetramethylbenzidine (TMB) as the chromogen. TMB forms crystalline reaction products that are very distinct at the electron microscopic level. The tissues were then processed for immunocytochemisty using an antiserum against SP. The chromogen used in this case, diaminobenzidine, yields amorphous reaction products. At the light microscopic level, labeled cells were observed primarily ipsilaterally in both intermediate and ventrolateral subdivisions of the hypoglossal nucleus. The majority of these labeled cells were seen at the level of obex. At the electron microscopic level, both asymmetric and symmetric synapses were observed. SP-immunoreactive nerve terminals formed asymmetric synapses with labeled dendrites and symmetric synapses with labeled perikarya. SP-labeled terminals also synapsed on unlabeled dendrites and somata. These are the firstultrastructural studies demonstrating synaptic interactions between hypoglossal motoneurons and SP terminals. These studies demonstrate that hypoglossal motoneurons that innervate intrinsic tongue muscles are modulated by SP and that SP may play a role in the control of fine movements of the tongue.

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Documento generato il 25/11/20 alle ore 08:34:35