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Titolo:
IMAGING EXOCYTOSIS AND ENDOCYTOSIS
Autore:
BETZ WJ; MAO F; SMITH CB;
Indirizzi:
UNIV COLORADO,SCH MED,DEPT PHYSIOL C240,4200 E 9TH AVE DENVER CO 80262 MOLEC PROBES INC EUGENE OR 97402
Titolo Testata:
Current opinion in neurobiology
fascicolo: 3, volume: 6, anno: 1996,
pagine: 365 - 371
SICI:
0959-4388(1996)6:3<365:IEAE>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG NEUROMUSCULAR-JUNCTION; MOTOR-NERVE TERMINALS; CHARGE-SHIFT PROBES; TRANSMITTER RELEASE; SYNAPTIC VESICLES; MECHANISM; MUSCLE; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
W.J. Betz et al., "IMAGING EXOCYTOSIS AND ENDOCYTOSIS", Current opinion in neurobiology, 6(3), 1996, pp. 365-371

Abstract

From the secretion of neurotransmitters via synaptic vesicles to the expulsion of cellular waste via contractile vacuoles, exocytosis and its sequel, endocytosis, are being explored with a variety of new optical tools. Fluorescent markers, especially styryl dyes such as FM1-43 (which reversibly labels endosomal membranes), have been used to followexo- and endocytic events in many cell types. Even though the development of new dyes is still largely empirical, some theoretical principles have emerged to guide future dye chemistry. Moreover, advances in optical imaging technology that augment conventional fluorescence microscopy are appearing. For example, interference reflection microscopy (which requires no flurophore) and total internal reflection microscopyhave recently been used to observe single exocytic events at the contact point between a glass coverslip and the plasma membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:25:17