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Titolo:
NMDA RECEPTOR ACTIVATION INHIBITS NEURONAL VOLUME REGULATION AFTER SWELLING-INDUCED BY VERATRIDINE-STIMULATED NA+ INFLUX IN RAT CORTICAL CULTURES
Autore:
CHURCHWELL KB; WRIGHT SH; EMMA F; ROSENBERG PA; STRANGE K;
Indirizzi:
HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT MED NEPHROL,CRIT CARE RES LABS,ENDERS 12 BOSTON MA 02115 HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT MED NEPHROL,CRIT CARE RES LABS BOSTON MA 02115 HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT ANESTHESIA,CRIT CARE RES LABS BOSTON MA 02115 HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT NEUROL BOSTON MA 02115 UNIV ARIZONA,DEPT PHYSIOL TUCSON AZ 85724
Titolo Testata:
The Journal of neuroscience
fascicolo: 23, volume: 16, anno: 1996,
pagine: 7447 - 7457
SICI:
0270-6474(1996)16:23<7447:NRAINV>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCITATORY AMINO-ACIDS; CELL-VOLUME; GLUTAMATE NEUROTOXICITY; EXTRACELLULAR-SPACE; DEPOLARIZING AGENTS; ASTROCYTE CULTURES; TAURINE RELEASE; NERVOUS-SYSTEM; TUBULE CELLS; CALCIUM;
Keywords:
OSMOREGULATION; EDEMA; EXCITOTOXICITY; MK-801;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
K.B. Churchwell et al., "NMDA RECEPTOR ACTIVATION INHIBITS NEURONAL VOLUME REGULATION AFTER SWELLING-INDUCED BY VERATRIDINE-STIMULATED NA+ INFLUX IN RAT CORTICAL CULTURES", The Journal of neuroscience, 16(23), 1996, pp. 7447-7457

Abstract

Neurons and glia experience rapid fluctuations in transmembrane solute and water fluxes during normal brain activity, Cell volume must be regulated under these conditions to maintain optimal neural function. Almost nothing is known, however, about how brain cells respond to volume challenges induced by changes in transmembrane solute flux, As such, we characterized the volume-regulatory mechanisms of cultured cortical neurons swollen by veratridine-stimulated Na+ influx: Exposure of cortical neurons to 100 mu M veratridine for 10-15 min caused a 1,8- to2-fold increase in cell volume that persisted for at least 90 min. This volume increase was blocked by extracellular Na+ removal or by exposure to 5 mu M tetrodotoxin, indicating that swelling is a result of Na+ entry via Na+ channels. Treatment of cells with veratridine together with various NMDA receptor antagonists had no effect on the magnitude of swelling. NMDA receptor antagonist-treated cells, however, underwent nearly complete volume recovery within 50-70 min after veratridineexposure. This recovery suggests that NMDA receptor activation disrupts neuronal osmoregulatory pathways. Volume regulation was blocked by Ba2-. quinidine, or 5-nitro-2-(3-phenylpropylamino) benzoic acid, indicating that swelling activates volume regulatory K+ and Cl- channels, Veratridine also caused a rapid, transient increase in intracellular Ca2+. Extracellular Ca2+ removal or intracellular Ca2+ chelation prevented or dramatically reduced veratridine-induced increases in intracellular Ca2+ and completely blocked volume recovery. These findings indicate that increases in Ca2+ during cell swelling induced by Na+ influx are required for activation of neuronal volume-regulatory pathways.

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Documento generato il 19/01/20 alle ore 06:14:06