Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NSO MYELOMA CELL-DEATH - INFLUENCE OF BCL-2 OVEREXPRESSION
Autore:
MURRAY K; ANG CE; GULL K; HICKMAN JA; DICKSON AJ;
Indirizzi:
UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PT LANCS ENGLAND
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 3, volume: 51, anno: 1996,
pagine: 298 - 304
SICI:
0006-3592(1996)51:3<298:NMC-IO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
APOPTOSIS; HYBRIDOMA; CULTURES; GENE; MECHANISMS; PREVENTION; RESISTANCE; SURVIVAL; KINETICS; DNA;
Keywords:
NOS; MYELOMA; APOPTOSIS; BCL-2; BAX; BCL-XL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
K. Murray et al., "NSO MYELOMA CELL-DEATH - INFLUENCE OF BCL-2 OVEREXPRESSION", Biotechnology and bioengineering, 51(3), 1996, pp. 298-304

Abstract

The productivity of recombinant mammalian cell lines grown in batch culture is often limited by the rapidity with which cells die on entry into the decline phase (the period of culture after the maximum cell density has been reached and where cell viability begins to fall). We examined the decline phase characteristics of the NSO myeloma cell linewith a view to modulating the cell death that ensues. Examination of nuclear morphology during culture revealed that the onset of the decline phase was marked by a time-dependent increase in the percentage of cells that exhibited condensed and fragmented nuclei. Furthermore, these changes coincided with a fall in DNA integrity. High molecular weight DNA appeared to be degraded into oligonucleosomal fragments. Taken together, these observations indicated that NSO cells die by the process of apoptosis. The protein encoded by the bcl-2 gene has been shown to counter apoptosis induced by a large variety of stimuli and in a number of different cell types, but is not expressed in NSO cells. We examined whether overexpression of this protein could prevent/ delay theonset of cell death seen during batch culture and also in response toserum limitation. Bcl-2 failed to affect the decline phase characteristics and serum dependence of NSO cells. In our search to explain these findings, we found that the NSO cell line expresses bar and also a high level of another Bcl-2 related protein, Bcl-x(L). Given that Bcl-X(L) is a sequence and functional homologue of Bcl-2, it is possible that Bcl-2 is redundant in the NSO cell background. These data thereforeindicate that cells such as NSO, which are used in biotechnologicallyimportant processes such as generation of hybridomas and expression of recombinant proteins, may express only a subset of genes important in apoptotic regulation. Modulation of the death characteristics of such cells will need to take account of the expression profile of such genes and their regulatory interactions. (C) 1996 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:45:53