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Titolo:
EFFECT OF TESTOSTERONE AND DIBUTYRYL-CAMP ON THE MEIOTIC COMPETENCE IN PIG OOCYTES OF VARIOUS SIZE CATEGORIES
Autore:
PETR J; TEPLA O; ROZINEK J; JILEK F;
Indirizzi:
RES INST ANIM PROD,DEPT REPROD BIOL CR-10400 PRAGUE 10 CZECH REPUBLIC PRAGUE AGR UNIV,DEPT VET SCI CR-16521 PRAGUE 6 CZECH REPUBLIC
Titolo Testata:
Theriogenology
fascicolo: 1, volume: 46, anno: 1996,
pagine: 97 - 108
SICI:
0093-691X(1996)46:1<97:EOTADO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE OOCYTES; INVITRO; MATURATION; FOLLICLES; GROWTH; BOVINE; CAMP;
Keywords:
PIG OOCYTE; MEIOTIC COMPETENCE; DBCAMP; TESTOSTERONE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
J. Petr et al., "EFFECT OF TESTOSTERONE AND DIBUTYRYL-CAMP ON THE MEIOTIC COMPETENCE IN PIG OOCYTES OF VARIOUS SIZE CATEGORIES", Theriogenology, 46(1), 1996, pp. 97-108

Abstract

Spontaneous meiosis resumption was investigated in small pig oocytes of various size categories. Two parameters were studied: 1) the possibility for inhibiting spontaneous meiosis resumption in small oocytes of various size categories and 2) the level of meiotic competence of small oocytes in which meiosis resumption had been temporarily blocked. It was observed that 1 mM dibutyryl cyclic adenosine monophosphate (dbcAMP) combined with 0.5 mu M testosterone were able to prevent spontaneous maturation in pig oocytes of various size categories cultured in vitro for up to 10 d. Moreover, a culture of these oocytes with dbcAMPand testosterone has a beneficial effect on the integrity of the oocyte-granulosa cell complex. When oocytes were allowed to mature after long-term inhibition of maturation, the percentage of oocytes able to resume maturation was significantly increased. However, the portion of oocytes which completed maturation, reaching the metaphase II (M II) stage, did not increase. It was found that dbcAMP, in combination with testosterone, also efficiently inhibited spontaneous maturation in fully grown pig oocytes. When the inhibitory effect of dbcAMP and testosterone was reversed in fully grown oocytes, maturation was significantly accelerated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:17:14