Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DETERMINATION OF THE PRIMARY STRUCTURE OF HOMOLOGOUS PROTEINS BY SEQUENCE-ANALYSIS OF PEPTIDE MIXTURES
Autore:
CAPORALE C; CARUSO C; BUONOCORE V;
Indirizzi:
UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM VITERBO ITALY
Titolo Testata:
Journal of protein chemistry
fascicolo: 4, volume: 15, anno: 1996,
pagine: 405 - 412
SICI:
0277-8033(1996)15:4<405:DOTPSO>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; COMPUTER-PROGRAM; ALPHA-AMYLASE; INHIBITOR;
Keywords:
PROTEIN SEQUENCE; MASS SPECTROMETRY; PEPTIDE MIXTURES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C. Caporale et al., "DETERMINATION OF THE PRIMARY STRUCTURE OF HOMOLOGOUS PROTEINS BY SEQUENCE-ANALYSIS OF PEPTIDE MIXTURES", Journal of protein chemistry, 15(4), 1996, pp. 405-412

Abstract

We propose a rapid method to determine the primary structure of a protein knowing the sequence of a homologous protein. The method consistsin submitting both the reduced and alkylated proteins to an enzymaticor chemical hydrolysis and performing the sequence analysis of the peptide mixtures. The assessment of the unknown sequence and the degree of identity of the two proteins are reached by comparing the two sequence analyses. The sequences of all the possible peptides present in the two mixtures are reconstructed and the differences in the two sequences are determined. If necessary, the differences can be confirmed by performing a mass spectrometric analysis of the two mixtures. We used this procedure on two homologous proteins of known sequence to furnishan application example of the method.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 03:04:36