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Titolo:
RAS P21 PROTEIN PROMOTES SURVIVAL AND DIFFERENTIATION OF HUMAN EMBRYONIC NEURAL CREST-DERIVED CELLS
Autore:
BORASIO GD; MARKUS A; HEUMANN R; CHEZZI C; SAMPIETRO A; WITTINGHOFER A; SILANI V;
Indirizzi:
UNIV MUNICH,KLINIKUM GROSSHADERN,NEUROL KLIN D-81366 MUNICH GERMANY RUHR UNIV BOCHUM,ABT MOL NEUROBIOCHEM D-44780 BOCHUM GERMANY UNIV MILAN,SCH MED,IRCCS,INST NEUROL,OSPED MAGGIORE I-20122 MILAN ITALY MAX PLANCK INST MOL PHYSIOL,ABT STRUKTURELLE BIOL D-44319 DORTMUND GERMANY
Titolo Testata:
Neuroscience
fascicolo: 4, volume: 73, anno: 1996,
pagine: 1121 - 1127
SICI:
0306-4522(1996)73:4<1121:RPPPSA>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; PC12 CELLS; SYMPATHETIC NEURONS; CHROMAFFIN CELLS; SENSORY NEURONS; MICROINJECTION; POPULATIONS; OUTGROWTH;
Keywords:
NERVE GROWTH FACTOR; SIGNAL TRANSDUCTION; DORSAL ROOT GANGLION NEURONS; SYMPATHETIC NEURONS; CHROMAFFIN CELLS; P75 LOW AFFINITY NGF RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
G.D. Borasio et al., "RAS P21 PROTEIN PROMOTES SURVIVAL AND DIFFERENTIATION OF HUMAN EMBRYONIC NEURAL CREST-DERIVED CELLS", Neuroscience, 73(4), 1996, pp. 1121-1127

Abstract

We have previously shown that the oncogene product p21Ras is essential for the survival and neurite outgrowth-promoting activity of nerve growth factor on cultured chick embryonic sensory, but not sympathetic neurons. In order to extend our observations to the human system and to non-neuronal cells, we introduced the oncogenic form of p21Ras into the cytoplasm of three different types of cultured human embryonic neural crest derivatives (8th-11th gestational week): dorsal root ganglion neurons, sympathetic neurons, and adrenal chromaffin cells. These cells are dependent on nerve growth factor for survival and/or fibre outgrowth in vitro. In dorsal root ganglion neurons, p21Ras promoted survival and fibre outgrowth which was quantitatively and qualitatively comparable to the nerve growth factor effect (84% vs. 95%, control 18%). Sympathetic neurons showed a similar effect, albeit with a higher background survival (91% vs. 93%, control 58%). On chromaffin cells, which respond to nerve growth factor with pronounced fibre outgrowth in culture, the effect of p21Ras was again comparable to that of nerve growth factor (35% vs. 30%, control 5%). The survival and fibre outgrowth-promoting effects of p21Ras on human embryonic dorsal root ganglion neurons, sympathetic neurons and chromaffin cells suggest an involvementof p21Ras in the intracellular signal transduction of nerve growth factor in human neural crest-derived cell populations. Copyright (C) 1996 IBRO. Published by Elsevier Science Ltd.

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Documento generato il 30/11/20 alle ore 00:21:56