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Titolo:
THE TRANSCRIPTION FACTOR PU.1 IS INVOLVED IN MACROPHAGE PROLIFERATION
Autore:
CELADA A; BORRAS FE; SOLER C; LLOBERAS J; KLEMSZ M; VANBEVEREN C; MCKERCHER S; MAKI RA;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT FISIOL IMMUNOL,AV DIAGONAL 645,CAMPUS BELLVITGE E-08028 BARCELONA SPAIN UNIV BARCELONA,FDN AUGUST PI & SUNYER E-08028 BARCELONA SPAIN INDIANA UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL INDIANAPOLIS IN 46202 SIDNEY KIMMEL CANC CTR SAN DIEGO CA 92121 LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
The Journal of experimental medicine
fascicolo: 1, volume: 184, anno: 1996,
pagine: 61 - 69
SICI:
0022-1007(1996)184:1<61:TTFPII>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; REGULATES EXPRESSION; HEMATOPOIETIC-CELLS; CD11B PROMOTER; ETS ONCOGENE; CHAIN GENE; RECEPTOR; PROTEIN; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
A. Celada et al., "THE TRANSCRIPTION FACTOR PU.1 IS INVOLVED IN MACROPHAGE PROLIFERATION", The Journal of experimental medicine, 184(1), 1996, pp. 61-69

Abstract

PU.1 is a tissue-specific transcription factor that is expressed in cells of the hematopoietic lineage including macrophages, granulocytes,and B lymphocytes. Bone marrow-derived macrophages transfected with an antisense PU.1 expression construct or treated with antisense oligonucleotides showed a decrease in proliferation compared with controls. In contrast, bone marrow macrophages transfected with a sense PU.1 expression construct displayed enhanced macrophage colony-stimulating factor (M-CSF)-dependent proliferation. Interestingly, there was no effect of sense or antisense constructs of PU.1 on the proliferation of theM-C:SF-independent cell line, suggesting that the response was M-CSF dependent. This was further supported by the finding that macrophages transfected with :I sense or an antisense PU.1 construct showed, respectively, an increased or a reduced level of surface expression of receptors for M-CSF. The enhancement of proliferation seems to be selective for PU.1, since transfections with several other members of the ets family, including ets -2 and fli-1, had no effect. Various mutants of PU.1 were also tested for their ability to affect macrophage proliferation. A reduction in macrophage proliferation was found when cells were transfected with a construct in which the DNA-binding domain of PU.1was expressed. The PEST (proline-, glutamic acid-, serine-, and threonine-rich region) sequence of the PU.1 protein, which is an important domain for protein-protein interactions in B cells, was found to have no influence on PU.1-enhanced macrophage proliferation when an expression construct containing PU.1 minus the PEST domain was transfected into bone marrow-derived macrophages. In vivo, PU.1 is phosphorylated onseveral serine residues. The transfection of plasmids containing PU.1with mutations at each of five serines showed that only positions 41 and 45 are critical for enhanced macrophage proliferation. We concludethat PU.1 is necessary for the M-CSF-dependent proliferation of macrophages. One of the proliferation-relevant targets of this transcription factor could be the M-CSF receptor.

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Documento generato il 12/07/20 alle ore 06:22:24