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Titolo:
DENITRIFICATION, A NOVEL TYPE OF RESPIRATORY METABOLISM IN FUNGAL MITOCHONDRION
Autore:
KOBAYASHI M; MATSUO Y; TAKIMOTO A; SUZUKI S; MARUO F; SHOUN H;
Indirizzi:
UNIV TSUKUBA,INST APPL BIOCHEM TSUKUBA IBARAKI 305 JAPAN UNIV TSUKUBA,INST APPL BIOCHEM TSUKUBA IBARAKI 305 JAPAN UNIV TSUKUBA,INST BIOL SCI TSUKUBA IBARAKI 305 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 27, volume: 271, anno: 1996,
pagine: 16263 - 16267
SICI:
0021-9258(1996)271:27<16263:DANTOR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRITE-INDUCIBLE CYTOCHROME-P-450; FUSARIUM-OXYSPORUM; NITRATE NITRITE; PARACOCCUS-DENITRIFICANS; ORIGINS; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
M. Kobayashi et al., "DENITRIFICATION, A NOVEL TYPE OF RESPIRATORY METABOLISM IN FUNGAL MITOCHONDRION", The Journal of biological chemistry, 271(27), 1996, pp. 16263-16267

Abstract

Subcellular localization and coupling to ATP synthesis were investigated with respect to the denitrifying systems of two fungi, Fusarium oxysporum and Cylindrocarpon tonkinense. Dissimilatory nitrate reductaseof F. oxysporum or nitrite reductase of C. tonkinense could be detected in the mitochondrial fraction prepared from denitrifying cells of each fungus. Fluorescence immunolocalization, cofractionation with mitochondrial marker enzymes, and cytochromes provided evidence that the denitrifying enzymes are co-purified with mitochondria. Respiratory substrates such as malate plus pyruvate, succinate, and formate were effective donors of electrons to these activities in the mitochondrial fractions, Moreover, nitrite and nitrate reduction were shown to be coupled to the synthesis of ATP with energy yields (P:NO3- or P:2e ratios) of 0.88 to 1.4, depending upon whether malate/pyruvate or succinate were provided as substrates, Nitrate or nitrite reductase activity was inhibited by inhibitors such as rotenone, antimycin A, and thenoyltrifluoroaeetone. Thus, fungal denitrification activities are localized to mitochondria and are coupled to the synthesis of ATP. The existence ofthese novel respiration systems are discussed with regard to the origin and evolution of mitochondria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:13:12