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Titolo:
INDUCIBLE NITRIC-OXIDE SYNTHASE, NITROTYROSINE, AND APOPTOSIS IN HELICOBACTER-PYLORI GASTRITIS - EFFECT OF ANTIBIOTICS AND ANTIOXIDANTS
Autore:
MANNICK EE; BRAVO LE; ZARAMA G; REALPE JL; ZHANG XJ; RUIZ B; FONTHAM ETH; MERA R; MILLER MJS; CORREA P;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT PATHOL,STANLEY SCOTT CANC CTR,1901 PERDIDO ST NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT PATHOL,STANLEY SCOTT CANC CTR NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT PEDIAT,STANLEY SCOTT CANC CTR NEW ORLEANS LA 70112 UNIV VALLE CALI COLOMBIA HOSP DEPT PASTO COLOMBIA
Titolo Testata:
Cancer research
fascicolo: 14, volume: 56, anno: 1996,
pagine: 3238 - 3243
SICI:
0008-5472(1996)56:14<3238:INSNAA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASCORBIC-ACID; INFECTION; CANCER; CELLS; CARCINOMA; RISK; NITROSATION; INVITRO; DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
E.E. Mannick et al., "INDUCIBLE NITRIC-OXIDE SYNTHASE, NITROTYROSINE, AND APOPTOSIS IN HELICOBACTER-PYLORI GASTRITIS - EFFECT OF ANTIBIOTICS AND ANTIOXIDANTS", Cancer research, 56(14), 1996, pp. 3238-3243

Abstract

Helicobacter pylori infection is a known risk factor for gastric cancer, We hypothesized that H. pylori infection would lead to the sustained production of the reactive nitrogen species nitric oxide and peroxynitrite as part of the host immune response, We further hypothesized that H. pylori infection would lead to increased apoptosis of gastric epithelial cells, possibly in response to free radical-mediated DNA damage, Using immunohistochemistry, we stained and scored gastric antral biopsies from 84 Colombian patients with nonatrophic gastritis before and after treatment for H. pylori infection, We examined expression ofinducible nitric oxide synthase (iNOS); nitrotyrosine, a marker for peroxynitrite; and DNA fragmentation, a marker for apoptosis, Patients were treated with triple therapy (amoxicillin, 500 mg three times a day for 2 weeks; metronidazole, 400 mg three times a day for 2 weeks; and bismuth subsalicylate, 262 mg four times a day for 2 weeks, followedby 262 mg every day for 4-12 months), Eradication of H. pylori infection resulted in a significant reduction in iNOS and nitrotyrosine staining and a marginally significant reduction in apoptosis. Dietary supplementation with beta-carotene (30 mg every day for 4-12 months) resulted in a significant decrease in iNOS staining, Supplementation with ascorbic acid (1 g twice a day for 4-12 months) led to a significant reduction in nitrotyrosine staining, In patients supplemented with either ascorbic acid or beta-carotene, there was a trend toward a reductionin apoptosis, but this was not statistically significant, We concludethat H. pylori infection is accompanied by the formation of endogenous reactive nitrogen intermediates, which may contribute to DNA damage and apoptosis, In addition to antimicrobial therapy, dietary supplementation with beta-carotene and ascorbic acid may prevent the formation of these potential carcinogens.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:31:09