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Titolo:
PRODUCTION OF A POLYHYDROXYALKANOATE BIOPOLYMER IN INSECT CELLS WITH A MODIFIED EUKARYOTIC FATTY-ACID SYNTHASE
Autore:
WILLIAMS MD; RAHN JA; SHERMAN DH;
Indirizzi:
BPTI,GORTNER LABS 240,1479 GORTNER AVE ST PAUL MN 55108 BPTI,GORTNER LABS 240 ST PAUL MN 55108 DEPT MICROBIOL,GORTNER LABS 240 ST PAUL MN 55108
Titolo Testata:
Applied and environmental microbiology
fascicolo: 7, volume: 62, anno: 1996,
pagine: 2540 - 2546
SICI:
0099-2240(1996)62:7<2540:POAPBI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLY-BETA-HYDROXYBUTYRATE; ALCALIGENES-EUTROPHUS H16; LIMITED TRYPSINIZATION; MOLECULAR-GENETICS; BIOSYNTHESIS; SYNTHETASE; COMPLEX; IDENTIFICATION; CONSTRUCTION; TRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
M.D. Williams et al., "PRODUCTION OF A POLYHYDROXYALKANOATE BIOPOLYMER IN INSECT CELLS WITH A MODIFIED EUKARYOTIC FATTY-ACID SYNTHASE", Applied and environmental microbiology, 62(7), 1996, pp. 2540-2546

Abstract

A novel pathway for the synthesis of poly-3-hydroxybutyrate has been engineered by simultaneous delivery of two genes into insect cells (Spodoptera frugiperda) by use of individual baculovirus vectors. This system includes expression of a dehydrase-domain mutant rat fatty acid synthase cDNA and the phbC gene encoding polyhydroxyalkanoate synthase from Alcaligenes eutrophus. The dehydrase-deficient fatty acid synthase provides de novo synthesis of R-(-)-3-hydroxybutyryl-coenzyme A as apremature termination product rather than palmityl-coenzyme A, the normal product of wild-type rat fatty acid synthase. High levels of thismutant multifunctional protein provide a suitable precursor pool of R-(-)-3-hydroxybutyryl-coenzyme A for conversion to poly-3-hydroxybutyrate in insect cells coexpressing the phbC gene product. This strategy for redesigning a poly-3-hydroxybutyrate biosynthetic pathway suggestsa new method for generating structurally diverse polyhydroxyalkanoates by metabolic engineering.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 01:59:40