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Titolo:
RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA SUPPRESSES SYNTHESIS, ACTIVITY AND SECRETION OF LIPOPROTEIN-LIPASE IN CULTURES OF A HUMAN OSTEOSARCOMA CELL-LINE
Autore:
SAKAYAMA K; MASUNO H; OKUMURA H; SHIBATA T; OKUDA H;
Indirizzi:
EHIME COLL HLTH SCI,DEPT MED LAB TECHNOL TOBE EHIME 79121 JAPAN EHIME COLL HLTH SCI,DEPT MED LAB TECHNOL TOBE EHIME 79121 JAPAN EHIME UNIV,SCH MED,DEPT ORTHOPAED SURG SHIGENOBU EHIME 79102 JAPAN EHIME UNIV,SCH MED,DEPT BIOCHEM MED SHIGENOBU EHIME 79102 JAPAN
Titolo Testata:
Biochemical journal
, volume: 316, anno: 1996,
parte:, 3
pagine: 813 - 817
SICI:
0264-6021(1996)316:<813:RHTSSA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR ANTIGEN; 3T3-L1 ADIPOCYTES; INTERFERON-GAMMA; BROWN ADIPOCYTES; FACTOR TNF; GLUCOSE; INVIVO; MICE; EXPRESSION; CACHECTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
K. Sakayama et al., "RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA SUPPRESSES SYNTHESIS, ACTIVITY AND SECRETION OF LIPOPROTEIN-LIPASE IN CULTURES OF A HUMAN OSTEOSARCOMA CELL-LINE", Biochemical journal, 316, 1996, pp. 813-817

Abstract

The effect of recombinant human tumour necrosis factor-alpha (TNF-alpha) on synthesis, activity and secretion of lipoprotein lipase (LPL) was examined using a human osteosarcoma cell line, osteosarcoma Takase (OST). Treatment of OST cells with TNF-alpha decreased LPL synthesis, resulting in a decrease in expression of activity and secretion of LPL. When OST cells were incubated with glycerol tri[1-C-14]palmitate, TNF-alpha decreased dose- and time-dependently the production of (CO2)-C-14 and the amounts of radioactivity incorporated into cellular triacylglycerol and phospholipid. The similar reduction of synthesis and activity of LPL as suppression of CO2 production and cellular lipid synthesis indicated that the suppression of (CO2)-C-14 production and C-14-labelled lipid synthesis was secondary. TNF-alpha also suppressed expression of proliferating cell nuclear antigen, indicating that it had an anti-proliferative activity on OST cells. The findings suggest that one cause of the anti-proliferative activity of TNF-alpha is the suppression of the LPL-mediated supply of non-esterified fatty acids as an energy source for growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 03:34:36