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Titolo:
SYNTHESIS AND PHYSIOLOGICAL-ACTIVITY OF HETERODIMERS COMPRISING DIFFERENT SPLICE FORMS OF VASCULAR ENDOTHELIAL GROWTH-FACTOR AND PLACENTA GROWTH-FACTOR
Autore:
BIRKENHAGER R; SCHNEPPE B; ROCKL W; WILTING J; WEICH HA; MCCARTHY JEG;
Indirizzi:
NATL BIOTECHNOL RES CTR,DEPT GENE EXPRESS,MASCHERODER WEG 1 D-38124 BRAUNSCHWEIG GERMANY NATL BIOTECHNOL RES CTR,DEPT GENE EXPRESS D-38124 BRAUNSCHWEIG GERMANY UNIV FREIBURG,INST ANAT 2 D-79104 FREIBURG GERMANY
Titolo Testata:
Biochemical journal
, volume: 316, anno: 1996,
parte:, 3
pagine: 703 - 707
SICI:
0264-6021(1996)316:<703:SAPOHC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERMEABILITY FACTOR; ESCHERICHIA-COLI; CELL MITOGEN; TYROSINE KINASE; HUMAN-PLATELETS; RECEPTOR; EXPRESSION; PDGF; PURIFICATION; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
R. Birkenhager et al., "SYNTHESIS AND PHYSIOLOGICAL-ACTIVITY OF HETERODIMERS COMPRISING DIFFERENT SPLICE FORMS OF VASCULAR ENDOTHELIAL GROWTH-FACTOR AND PLACENTA GROWTH-FACTOR", Biochemical journal, 316, 1996, pp. 703-707

Abstract

Vascular endothilial growth factor (VEGF) and placenta growth factor (PIGF) are members of a dimeric-growth-factor family with angiogenic properties. VEGF is a highly potent and specific mitogen for endothelial cells, playing a vital role in angiogenesis in vivo. The role of PIGF is less clear. We expressed the monomeric splice forms VEGF-165, VEGF-121, PIGF-1 and PIGF-2 as unfused genes in Escherichia coli using the pCYTEXP expression system. In vitro dimerization experiments revealed that both homo- and hetero-dimers can be formed from these monomericproteins. The dimers were tested for their ability to promote capillary growth in vivo and stimulate DNA synthesis in cultured human vascular endothelial cells. Heterodimers comprising different VEGF splice forms, or combinations of VEGF/PIGF splice forms, showed mitogenic activity. The results demonstrate that four different heterodimeric growth factors are likely to have as yet uncharacterized functions in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:19:52