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Titolo:
BINDING-SITES FOR MAIZE NUCLEAR PROTEINS IN THE SUBTERMINAL REGIONS OF THE TRANSPOSABLE ELEMENT ACTIVATOR
Autore:
BECKER HA; KUNZE R;
Indirizzi:
UNIV MUNICH,INST MIKROBIOL & GENET,MARIA WARD STR 1A D-80638 MUNCHEBERG GERMANY UNIV MUNICH,INST MIKROBIOL & GENET D-80638 MUNCHEBERG GERMANY UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 4, volume: 251, anno: 1996,
pagine: 428 - 435
SICI:
0026-8925(1996)251:4<428:BFMNPI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTEGRATION HOST FACTOR; TRANSPOSITION INVITRO PROCEEDS; ZEA-MAYS-L; TN7 TRANSPOSITION; BACTERIOPHAGE-MU; ATTACHMENT SITE; DNA; AC; TRANSCRIPTION; DROSOPHILA;
Keywords:
TRANSPOSABLE ELEMENT; AC; ZEA MAYS; HOST FACTOR; DNASE I PROTECTION ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
H.A. Becker e R. Kunze, "BINDING-SITES FOR MAIZE NUCLEAR PROTEINS IN THE SUBTERMINAL REGIONS OF THE TRANSPOSABLE ELEMENT ACTIVATOR", MGG. Molecular & general genetics, 251(4), 1996, pp. 428-435

Abstract

Genetic data suggest that transposition of the maize element Activator (Ac) is modulated by host factors. Using gel retardation and DNase Iprotection assays we identified maize proteins which bind to seven subterminal sires in both ends of Ac. Four DNase I-protected sites contain a GGTAAA sequence, the other three include either GATAAA or GTTAAA. The specificity of the maize protein binding to Ac was verified by using a synthetic fragment containing four GGTAAA motifs as probe and competitor in gel retardation assays. All seven binding sites are located within regions required in cis for transposition. A maize protein binding site with the same sequence has previously been identified in the terminal inverted repeats of the maize Mutator element. Thus, the protein, that recognizes this sequence is a good candidate for a regulatory host factor for Ac transposition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:29:21