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Titolo:
EFFECT OF DIFFERENTIAL GENE-EXPRESSION ON THE CHROMATIN STRUCTURE OF THE DHFR GENE DOMAIN IN-VIVO
Autore:
LJUNGMAN M;
Indirizzi:
UNIV MICHIGAN,CTR MED,DIV CANC BIOL,DEPT RADIAT ONCOL,1331 E ANN ST ANN ARBOR MI 48109
Titolo Testata:
Biochimica et biophysica acta, N. Gene structure and expression
fascicolo: 2, volume: 1307, anno: 1996,
pagine: 171 - 177
SICI:
0167-4781(1996)1307:2<171:EODGOT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIHYDROFOLATE-REDUCTASE GENE; 5,6-DICHLORO-1-BETA-D-RIBOFURANOSYLBENZIMIDAZOLE INHIBITS TRANSCRIPTION; DNA TOPOISOMERASE-I; HAMSTER OVARY CELLS; HEAT-SHOCK GENES; BETA-GLOBIN GENE; HYPERSENSITIVE SITES; TORSIONAL TENSION; SENSITIVE DOMAIN; PROMOTER REGION;
Keywords:
TRANSCRIPTION; DIHYDROFOLATE REDUCTASE GENE; PSORALEN; CAMPTOTHECIN; 5,6-DICHLORO-1-BETA-D-RIBOFURANOSYL-BENZIMIDAZOLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
M. Ljungman, "EFFECT OF DIFFERENTIAL GENE-EXPRESSION ON THE CHROMATIN STRUCTURE OF THE DHFR GENE DOMAIN IN-VIVO", Biochimica et biophysica acta, N. Gene structure and expression, 1307(2), 1996, pp. 171-177

Abstract

Photoactivated psoralen was used to probe region-specific chromatin structure in Chinese hamster ovary (CHO) cells. Specifically, the chromatin structure of six regions within the dihydrofolate reductase (DHFR) gene was probed with photoactivated psoralen in cells cultured in such ways as to differentially express the DHFR gene. Cells were irradiated with X-rays prior to the psoralen photocross-linking reaction in order to eliminate the influence of any DNA torsional tension on the psoralen binding and the sequence-specificity of psoralen binding was adjusted for. It was found that a region encompassing the promoter of the serum-regulated DHFR gene was about 50% more accessible to psoralen photocross-linking in serum-stimulated cells and about 90% more accessible in serum-starved cells than the other five regions of the DHFR gene analyzed and the genome overall. Treating serum-stimulated cells with the RNA polymerase II transcriptional inhibitor 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB) or the topoisomerase I inhibitor camptothecin reversed the elevated accessibility of the DHFR promoter region. These results suggest that the accessible chromatin structure of the DHFR promoter is not dependent on serum-stimulated poising of the gene for transcription, but may reflect the ability of the RNA polymerase to clear the promoter.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:36:50