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Titolo:
WGA LECTIN-BINDING SITES OF THE APICAL SURFACE OF CORTI EPITHELIUM - ENHANCEMENT BY BACKSCATTERED ELECTRON IMAGING IN GUINEA-PIG INNER-EAR
Autore:
KATORI Y; TONOSAKI A; TAKASAKA T;
Indirizzi:
TOHOKU UNIV,SCH MED,DEPT OTOLARYNGOL SENDAI MIYAGI 980 JAPAN YAMAGATA UNIV,SCH MED,DEPT ANAT YAMAGATA 99023 JAPAN
Titolo Testata:
Journal of Electron Microscopy
fascicolo: 3, volume: 45, anno: 1996,
pagine: 207 - 212
SICI:
0022-0744(1996)45:3<207:WLSOTA>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHEAT-GERM AGGLUTININ; COCHLEAR HAIR-CELLS; COLLOIDAL GOLD; COAT; STEREOCILIA; MICROSCOPY; ORGAN;
Keywords:
CORTI EPITHELIUM; HAIR CELL; WGA LECTIN; SEM; BACKSCATTERED ELECTRON IMAGING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
Y. Katori et al., "WGA LECTIN-BINDING SITES OF THE APICAL SURFACE OF CORTI EPITHELIUM - ENHANCEMENT BY BACKSCATTERED ELECTRON IMAGING IN GUINEA-PIG INNER-EAR", Journal of Electron Microscopy, 45(3), 1996, pp. 207-212

Abstract

The apical surface of the guinea-pig organ of Corti was examined withSEM after WGA-lectin histochemistry which has a binding specificity for sialic acid and N-acetyl-glucosamine. The contrast of colloidal gold markers were particularly enhanced by back-scattered electron imaging. WGA lectin showed remarkable preference for the microvilli as well as flat surface areas in the supporting cells, Stereociliary surfaces and interstereociliary connections of the sensory hair cell were strongly labelled, whilst only rarely the markers were found in the flat areas of the apical surface of the hair cell. The connections, which areknown to be involved in the initiation of the mechanoelectrical transduction, should consist of sialic acid and/or N-acetyl-glucosamine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 06:41:05