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Titolo:
HUMAN COLON-CARCINOMA CELLS SYNTHESIZE AND SECRETE ALPHA(1)-PROTEINASE INHIBITOR
Autore:
KEPPLER D; MARKERT M; CARNAL B; BERDOZ J; BAMAT J; SORDAT B;
Indirizzi:
WAYNE STATE UNIV,SCH MED,DEPT PHARMACOL,540 E CANFIELD,6304 SCOTT HALL DETROIT MI 48201 SWISS INST EXPTL CANC RES,EXPTL PATHOL UNIT CH-1066 EPALINGES SWITZERLAND UNIV LAUSANNE HOSP,CENT LAB CLIN CHEM CH-1002 LAUSANNE SWITZERLAND SWISS INST EXPTL CANC RES CH-1066 EPALINGES SWITZERLAND
Titolo Testata:
Biological chemistry Hoppe-Seyler
fascicolo: 5, volume: 377, anno: 1996,
pagine: 301 - 311
SICI:
0177-3593(1996)377:5<301:HCCSAS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATHEPSIN-B; LEUKOCYTE ELASTASE; PLASMINOGEN ACTIVATORS; TUMOR INVASION; BREAST-CANCER; CYSTATIN-C; METASTASIS; EXPRESSION; PROTEINASE; GENE;
Keywords:
ALPHA(1)-ANTITRYPSIN; CATHEPSIN B; ELASTASE; NEUTROPHILS; POLYMORPHONUCLEAR LEUKOCYTES; TUMOR INVASION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
D. Keppler et al., "HUMAN COLON-CARCINOMA CELLS SYNTHESIZE AND SECRETE ALPHA(1)-PROTEINASE INHIBITOR", Biological chemistry Hoppe-Seyler, 377(5), 1996, pp. 301-311

Abstract

Our previous results have shown that tumor cell-secreted procathepsinB can be activated by neutrophil elastase in vitro. In the present study, we addressed two questions: 1. Can neutrophil elastase be detected in human colon carcinomas, and 2. Does the co-culture of human coloncarcinoma cells with neutrophils generate a cathepsin B-dependent pericellular proteolysis as assessed with radiolabeled laminin? We show that neutrophil elastase is present in colon carcinoma tissue and that its level is in good agreement with the degree of tissue infiltration by neutrophils. In co-culture experiments, elastase is released by neutrophils in a cell number dependent way, but no activation of tumor cell-secreted procathepsin B could be observed. In addition, the degradation of radiolabeled laminin by neutrophil proteinases was markedly decreased in the presence of tumor cells. These findings prompted us to search for a tumor cell-secreted elastase inhibitor. We show by enzymeactivity measurements, gelatin-zymography, immuno-blotting and RT-PCRthat colon carcinoma cells synthesize and secrete alpha(1)-proteinaseinhibitor, a functional inhibitor of neutrophil elastase. The importance of this finding in the context of pericellular activation of tumorcell-secreted procathepsin B by neutrophil elastase is discussed.

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Documento generato il 10/07/20 alle ore 00:21:29