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Titolo:
IN THE ABSENCE OF ENDOGENOUS IL-1O, MICE ACUTELY INFECTED WITH TOXOPLASMA-GONDII SUCCUMB TO A LETHAL IMMUNE-RESPONSE DEPENDENT ON CD4(-CELLS AND ACCOMPANIED BY OVERPRODUCTION OF IL-12, IFN-GAMMA, AND TNF-ALPHA() T)
Autore:
GAZZINELLI RT; WYSOCKA M; HIENY S; SCHARTONKERSTEN T; CHEEVER A; KUHN R; MULLER W; TRINCHIERI G; SHER A;
Indirizzi:
NIAID,IMMUNOBIOL SECT,PARASIT DIS LAB,NIH,BLDG 4,ROOM 126 BETHESDA MD20892 NIAID,IMMUNOBIOL SECT,PARASIT DIS LAB,NIH BETHESDA MD 20892 WISTAR INST ANAT & BIOL,LAB HUMAN IMMUNOL PHILADELPHIA PA 19104 UNIV COLOGNE,INST GENET W-5000 COLOGNE GERMANY UNIV FED MINAS GERAIS,DEPT BIOCHEM & IMMUNOL BELO HORIZONT MG BRAZIL
Titolo Testata:
The Journal of immunology
fascicolo: 2, volume: 157, anno: 1996,
pagine: 798 - 805
SICI:
0022-1767(1996)157:2<798:ITAOEI>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; INTERLEUKIN-10 PROTECTS MICE; INHIBITS CYTOKINE PRODUCTION; NATURAL-KILLER-CELLS; INTERFERON-GAMMA; ACTIVATED MACROPHAGES; TRYPANOSOMA-CRUZI; ACCESSORY CELLS; LYMPHOCYTES-T; TH1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
R.T. Gazzinelli et al., "IN THE ABSENCE OF ENDOGENOUS IL-1O, MICE ACUTELY INFECTED WITH TOXOPLASMA-GONDII SUCCUMB TO A LETHAL IMMUNE-RESPONSE DEPENDENT ON CD4(-CELLS AND ACCOMPANIED BY OVERPRODUCTION OF IL-12, IFN-GAMMA, AND TNF-ALPHA() T)", The Journal of immunology, 157(2), 1996, pp. 798-805

Abstract

To examine the function of IL-10 synthesis during early infection with the intracellular protozoan Toxoplasma gondii, IL-10 knockout (KO) mice were inoculated with an avirulent parasite strain (ME-49). In contrast to control littermates that displayed 100% survival, the IL-10-deficient animals succumbed within the first 2 wk of the infection, withno evidence of enhanced parasite proliferation. The mortality in the IL-10 KO mice was associated with enhanced liver pathology characterized by increased cellular infiltration and intense necrosis. Levels of IL-12 and IFN-gamma in sera of infected IL-10-deficient animals were four- to sixfold higher than those in sera from control mice, as were mRNA levels for IFN-gamma, IL-1 beta, TNF-alpha, and IL-12 in lung tissue. Similarly, macrophages from IL-10 KO mice activated in vitro or invivo with T. gondii produced higher levels of TNF-alpha and IL-12 than macrophages from control animals. Moreover, spleen cells from IL-10 KO mice infected with T. gondii secreted more IFN-gamma than splenocytes from nondeficient animals. In vitro depletion experiments indicatedthat CD4(+) lymphocytes are the major source of the latter cytokine in the spleen cell populations, and in vivo depletion with anti-CD4 Absprotected the IL-10 KO mice from parasite-induced mortality. Togetherthe data suggest that endogenous IL-10 synthesis plays an important role in vivo in down-regulating monokine and IFN-gamma responses to acute intracellular infection, thereby preventing host immunopathology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:47:01