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Titolo:
ISOLATION AND CHARACTERIZATION OF A HOMEODOMAIN-LEUCINE ZIPPER GENE, GMH1, FROM SOYBEAN SOMATIC EMBRYO
Autore:
MOON YH; CHOI D; KIM JC; HAN TJ; CHO SH; KIM WT; LEE KW;
Indirizzi:
SEOUL NATL UNIV,DEPT BIOL SEOUL 151742 SOUTH KOREA SEOUL NATL UNIV,DEPT BIOL SEOUL 151742 SOUTH KOREA KANGWEON NATL UNIV,DEPT BIOL CHUNCHON 200701 SOUTH KOREA HALLYM UNIV,DEPT BIOL CHOONCHUN 200702 SOUTH KOREA INHA UNIV,DEPT BIOL INCHON 402751 SOUTH KOREA YONSEI UNIV,DEPT BIOL SEOUL 120749 SOUTH KOREA
Titolo Testata:
Molecules and cells
fascicolo: 3, volume: 6, anno: 1996,
pagine: 366 - 373
SICI:
1016-8478(1996)6:3<366:IACOAH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING PROTEINS; MAIZE; ANTENNAPEDIA; SPECTROSCOPY; ACTIVATORS; COMPLEXES; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
Y.H. Moon et al., "ISOLATION AND CHARACTERIZATION OF A HOMEODOMAIN-LEUCINE ZIPPER GENE, GMH1, FROM SOYBEAN SOMATIC EMBRYO", Molecules and cells, 6(3), 1996, pp. 366-373

Abstract

By screening a soybean somatic embryo cDNA library with a Arabidopsisthaliana homeo-domain-leucine zipper (HE-Zip) cDNA clone as a probe, ave isolated a cDNA clone, Glycine mas homeobox gene 1 (Gmh1), encoding a polypeptide which contains a homeodomain and a closely linked leucine zipper motif, In both nucleotide and deduced amino acid sequences,Gmh1 was highly homologous to two Arabidopsis thaliana HD-Zip genes and a tomato HD-Zip gene in HD-Zip motif. Genomic Southern blot analysis indicated that Gmh1 is a single copy gene or a member of a small gene family. Also, genomic DNA of Gmh1 has three introns, 242 bp, 261 bp and 66 bp long, respectively, in the coding region. RT-PCR and Southern hybridization revealed that Gmh1 is expressed at all organs including stems, leaves, roots, flowers, seeds and somatic embryos. These results suggest that Gmh1 protein may act as a transcriptional activator during somatic embryogenesis and seed development as well as during thedevelopment of other organs in soybean.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:05:55