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Titolo:
LECTIN AFFINITY ELECTROPHORESIS OF ALPHA-FETOPROTEIN DETECTED BY IMMUNO-ENZYMATIC AND CHEMILUMINESCENT AMPLIFICATION FOLLOWED BY DIRECT SCANNING
Autore:
OGATA M; TAGA H; TAKETA K;
Indirizzi:
OKAYAMA UNIV,SCH MED,DEPT PUBL HLTH OKAYAMA 700 JAPAN TUMOR LABS TOKYO 185 JAPAN
Titolo Testata:
Acta medica Okayama
fascicolo: 3, volume: 50, anno: 1996,
pagine: 119 - 124
SICI:
0386-300X(1996)50:3<119:LAEOAD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATOCELLULAR-CARCINOMA;
Keywords:
ALPHA-FETOPROTEIN; LECTIN AFFINITY ELECTROPHORESIS; CHEMILUMINESCENCE; ANTIBODY-AFFINITY BLOTTING; AVIDIN-BIOTIN COMPLEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
9
Recensione:
Indirizzi per estratti:
Citazione:
M. Ogata et al., "LECTIN AFFINITY ELECTROPHORESIS OF ALPHA-FETOPROTEIN DETECTED BY IMMUNO-ENZYMATIC AND CHEMILUMINESCENT AMPLIFICATION FOLLOWED BY DIRECT SCANNING", Acta medica Okayama, 50(3), 1996, pp. 119-124

Abstract

Microdetermination of a-fetoprotein (AFP) glycoforms by lectin affinity electrophoresis followed by chemiluminescence reaction using horseradish peroxidase (POD) or alkaline phosphatase (ALP) in antibody-affinity blotting was developed. The intensity of chemiluminescence obtained by ALP was greater than that by POD; however, the coefficient of variation with POD was less than that with ALP. The optimized sensitivityof the chemiluminescence method with POD was two times that of the most sensitive colorimetric method currently available in terms of the chemiluminescence intensity per unit AFP concentration. The lower detection limit by the chemiluminescence method with POD (0.5 ng/ml) was much lower than that by the colorimetric method (3 ng/ml). Both methods gave identical percentages of lentil lectin- and erythroagglutinating phytohemagglutinin-reactive minor bands using a serum with 52 ng/ml AFP. This result indicates that microdetermination of AFP glycoforms by chemiluminescence after lectin-affinity electrophoresis was more sensitive than currently available methods and that it is potentially useful for clinical application.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 11:59:24