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Titolo:
AMYLASE GENE STRUCTURES IN PRIMATES - RETROPOSON INSERTIONS AND PROMOTER EVOLUTION
Autore:
SAMUELSON LC; PHILLIPS RS; SWANBERG LJ;
Indirizzi:
UNIV MICHIGAN,DEPT PHYSIOL,7761 MED SCI II ANN ARBOR MI 48109
Titolo Testata:
Molecular biology and evolution
fascicolo: 6, volume: 13, anno: 1996,
pagine: 767 - 779
SICI:
0737-4038(1996)13:6<767:AGSIP->2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SALIVARY; ACTIN PSEUDOGENE; EXPRESSION; DNA; PROTEIN; SEQUENCES; ADJACENT; FAMILY; LOCUS; GAMMA;
Keywords:
ALPHA-AMYLASE; MULTIGENE FAMILY; PROMOTERS; MOLECULAR EVOLUTION; PRIMATES; TRANSPOSABLE ELEMENTS; RETROVIRUSES; TISSUE-SPECIFIC GENE EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
L.C. Samuelson et al., "AMYLASE GENE STRUCTURES IN PRIMATES - RETROPOSON INSERTIONS AND PROMOTER EVOLUTION", Molecular biology and evolution, 13(6), 1996, pp. 767-779

Abstract

Amylase transcription in the human salivary gland results from the evolutionary juxtaposition of two inserted elements, a gamma-actin pseudogene and an endogenous retrovirus, to create an unusual salivary-specific promoter. We utilized these structures as molecular tags to characterize the amylase genes in extant primates by polymerase chain reaction amplification of promoter fragments from genomic DNA. Six distinctamylase promoter structures were identified, which allowed us to infer the structures of common ancestors and trace the evolution of the modem human amylase promoters. Our data show that integration of the pseudogene and retrovirus were evolutionarily recent events. The gamma-actin pseudogene integrated after the divergence of the New World monkeys from the primate ancestral tree, and the retrovirus integrated later, after the divergence of the Old World monkeys. The New World monkey amylase promoter represents the mammalian amylase precursor structure before integration of the two retroposons. Two distinct amylase genes were identified in the Old World monkeys, one with a complete gamma-actin pseudogene insert and another novel structure with a truncation ofthe gamma-actin sequences. We demonstrated abundant amylase expression in the saliva of an Old World monkey, indicating that the endogenousretrovirus is not required for amylase transcription in the primate salivary gland.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 13:57:25