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Titolo:
A LIGHT-SCATTERING ASSAY FOR LYMPHOCYTE SHAPE AND ITS APPLICATION TO T-LYMPHOCYTE AND B-LYMPHOCYTE RESPONSES TO CULTURED HIGH-WALLED ENDOTHELIAL-CELLS
Autore:
HARRIS H; JOLLEY C; MILLER NG;
Indirizzi:
BABRAHAM INST,DEPT IMMUNOL CAMBRIDGE CB2 4AT ENGLAND
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 192, anno: 1996,
pagine: 179 - 185
SICI:
0022-1759(1996)192:1-2<179:ALAFLS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-BLOOD LYMPHOCYTES; LOCOMOTOR CAPACITY; RAT THYMUS; ALPHA-4-BETA-1; MIGRATION; ADHESION; ANTIGEN; IA;
Keywords:
FLOW CYTOMETRY; LYMPHOCYTE; HIGH-WALLED ENDOTHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
H. Harris et al., "A LIGHT-SCATTERING ASSAY FOR LYMPHOCYTE SHAPE AND ITS APPLICATION TO T-LYMPHOCYTE AND B-LYMPHOCYTE RESPONSES TO CULTURED HIGH-WALLED ENDOTHELIAL-CELLS", Journal of immunological methods, 192(1-2), 1996, pp. 179-185

Abstract

Lymphocyte shape changes are detectable as changes in the forward light scatter (FLS) profile of a Becton-Dickinson FACStar flow cytometer,with polar cells giving lower values of FLS. The FACScan cell analyser does not detect these changes. Freshly isolated lymph node lymphocytes are round, but when stimulated, for example by incubation with cultured high endothelial cells (HEC), a proportion change shape and become polar. The FLS profiles of lymphocytes stained for T and B cell surface markers show that both subsets change shape in response to HEC, but in a consistently different manner. Light microscopy of sorted T andB populations indicates that equal proportions of both cell types change shape, but that T cells are more likely to become highly elongated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 05:59:54