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Titolo:
ASTROGLIA-RELEASED FACTOR WITH NEGATIVE ALLOSTERIC MODULATORY PROPERTIES AT THE GABA(A) RECEPTOR
Autore:
RIGO JM; BELACHEW S; COUCKE P; LEPRINCE P; MALGRANGE B; ROGISTER B; MOONEN G;
Indirizzi:
UNIV LIEGE,LAB HUMAN PHYSIOL & PATHOPHYSIOL,PL DELCOUR 17 B-4020 LIEGE 2 BELGIUM UNIV LIEGE,LAB HUMAN PHYSIOL & PATHOPHYSIOL B-4020 LIEGE 2 BELGIUM
Titolo Testata:
Biochemical pharmacology
fascicolo: 3, volume: 52, anno: 1996,
pagine: 465 - 473
SICI:
0006-2952(1996)52:3<465:AFWNAM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HIPPOCAMPAL-NEURONS; AMINOBUTYRIC-ACID RECEPTORS; S-35 TBPS BINDING; BENZODIAZEPINE RECEPTORS; FLUNITRAZEPAM BINDING; INDUCED CURRENTS; A RECEPTORS; BRAIN; EXPRESSION; DESENSITIZATION;
Keywords:
GABA(A) RECEPTOR; NEGATIVE ALLOSTERIC MODULATOR; ASTROGLIA; BINDING; PATCH-CLAMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Rigo et al., "ASTROGLIA-RELEASED FACTOR WITH NEGATIVE ALLOSTERIC MODULATORY PROPERTIES AT THE GABA(A) RECEPTOR", Biochemical pharmacology, 52(3), 1996, pp. 465-473

Abstract

We have previously shown, using whole cell patch clamp techniques, that astrocytes release a negative allosteric modulator of the gamma-aminobutyric acid type A receptor (GABA(A) receptor) with beta-carboline-like properties, thus, likely to act at the benzodiazepine site. Here,using patch-clamp and binding techniques, we confirm that the low-molecular-weight fraction of astroglia-conditioned medium (ACM Imf) contains a factor(s) that negatively modulates GABA(A)-receptor function. This factor, like beta-carbolines, enhances the specific binding of [S-35]t-butyl bicyclophosphorothionate (TBPS) to adult rat cortical membranes in the presence of GABA. However, it fails to interact with various ligands of the benzodiazepine (BZD) site of the GABA(A) receptor ([H-3]flunitrazepam, [H-3]Ro 15-1788 and [H-3]Ro 15-4513). The question of the actual binding site of the astroglia-derived factor on the GABA(A) receptor, thus, remains open and can be addressed only after the purification of the active molecule(s) of ACM Imf has been completed, and a labeled form of the endogenous ligand becomes available. Taken together, however, the data suggest that type 1 astrocytes are able to modulate the effects of the main inhibitory neurotransmission in the central nervous system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:16:27