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Titolo:
CHANGES IN BINDING OF LECTINS TO EPIDIDYMAL, EJACULATED, AND CAPACITATED SPERMATOZOA OF THE RHESUS-MONKEY
Autore:
NAVANEETHAM D; SIVASHANMUGAM P; RAJALAKSHMI M;
Indirizzi:
ALL INDIA INST MED SCI,DEPT REPROD BIOL NEW DELHI 110029 INDIA ALL INDIA INST MED SCI,DEPT REPROD BIOL NEW DELHI 110029 INDIA
Titolo Testata:
The Anatomical record
fascicolo: 3, volume: 245, anno: 1996,
pagine: 500 - 508
SICI:
0003-276X(1996)245:3<500:CIBOLT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVITRO CAPACITATION; CYPROTERONE-ACETATE; SURFACE-ANTIGENS; CHIMPANZEE SPERM; PLASMA-MEMBRANE; MATURATION; SITES; RECEPTORS; RAT; ORGANIZATION;
Keywords:
LECTINS; SPERMATOZOA; EPIDIDYMIS; MONKEY; CAPACITATION; SPERM MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
D. Navaneetham et al., "CHANGES IN BINDING OF LECTINS TO EPIDIDYMAL, EJACULATED, AND CAPACITATED SPERMATOZOA OF THE RHESUS-MONKEY", The Anatomical record, 245(3), 1996, pp. 500-508

Abstract

Background: The present study was undertaken to evaluate the changes in rhesus monkey sperm surface glycoconjugates during maturation, ejaculation, and capacitation in order to provide background information that would help in evaluating adverse effects, if any, caused by the use of contraceptive agents. Methods: Adult sexually mature rhesus monkeys were castrated under ketamine anaesthesia, Percoll purified sperm from different epididymal segments and motile-ejaculated spermatozoa prior to and following in vitro capacitation were exposed to FITC-labeled lectins, Live or sperm prefixed with paraformaldehyde/alcohol were used, Quantitative analysis of changes in lectin binding was done by immunoassay. Results: The majority of live spermatozoa did not show binding of Con A and PNA, whereas uniform labeling of WGA to sperm from corpus epididymidis onward was seen, Unfixed spermatozoa showed marked variation in the pattern of lectin binding, The majority of spermatozoafixed with paraformaldehyde or alcohol showed maximum lectin labelingover the acrosome, but the postacrosomal area invariably did not bindthe lectins, Major changes in the localization of Con A, PNA, or WGA to prefixed spermatozoa were not seen during epididymal transit and inthe ejaculate, In capacitated acrosome-reacted spermatozoa, Con A, PNA, and WGA were localized mainly in the equatorial segment, Binding ofCon A and PNA was abolished by using lectins preincubated with appropriate inhibitor saccharides, Sperm, exposed to FITC-WGA preincubated with inhibitor sugar, did not show complete inhibition. Quantitative analysis of lectin binding by immunoassay showed increase in binding of lectins during epididymal transit with maximum binding in sperm from the corpus epididymidis. Conclusions: The variations in lectin labelingof live spermatozoa could be due to redistribution of sperm surface sugars or membrane damage. The changes in lectin labeling during maturation and capacitation may be associated with their role in ovum recognition and fusion. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:34:44