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Titolo:
EFFECT OF PROTOPORPHYRIN-IX LIMITATION ON PORPHYROMONAS-GINGIVALIS
Autore:
SCHIFFERLE RE; SHOSTAD SA; BAYERSTHERING MT; DYER DW; NEIDERS ME;
Indirizzi:
SUNY BUFFALO,DEPT ORAL BIOL & PERIODONTOL,318 FOSTER HALL BUFFALO NY 14214 SUNY BUFFALO,DEPT ENDODONT BUFFALO NY 14214 SUNY BUFFALO,DEPT STOMATOL & INTERDISCIPLINARY STUDIES BUFFALO NY 14214 UNIV OKLAHOMA,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL OKLAHOMA CITY OK 73190
Titolo Testata:
Journal of endodontics
fascicolo: 7, volume: 22, anno: 1996,
pagine: 352 - 355
SICI:
0099-2399(1996)22:7<352:EOPLOP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; IRON TRANSPORT PROTEINS; MEMBRANE-PROTEINS; GROWTH; PORPHYROMONAS-(BACTEROIDES)-GINGIVALIS; PORPHYRINS; VIRULENCE; HEMIN; W50;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
R.E. Schifferle et al., "EFFECT OF PROTOPORPHYRIN-IX LIMITATION ON PORPHYROMONAS-GINGIVALIS", Journal of endodontics, 22(7), 1996, pp. 352-355

Abstract

Porphyromonas gingivalis has been shown to require hemin or hemoglobin for in vitro growth. We have previously shown that protoporphyrin IXand inorganic iron can replace the hemin requirement, suggesting thatthe hemin requirement of this microorganism is actually a porphyrin requirement, We examined the effect of protoporphyrin IX limitation to P. gingivalis strain A7A1-28 in the presence of sufficient iron on growth characteristics, proteolytic enzyme production, virulence in a mouse abscess model, and expression of membrane proteins, Bacterial cellswere grown in medium varying between 0 to 5 mu M protoporphyrin IX, Protoporphyrin IX levels <0.25 mu M reduced growth by at least 50%. Protoporphyrin IX availability did not affect proteolytic enzyme production or virulence in a mouse abscess model, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of membrane preparations demonstrated that protoporphyrin IX limitation induced the expression of new proteinsat 42, 34, 30, 29, and 18 kDa and suppressed the production of proteins at 47, 27, 17, and 15 kDa, These studies suggest that in vivo protoporphyrin availability may modulate membrane protein expression and inturn affect host immune responses against P. gingivalis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:16:53