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Titolo:
AGE-RELATED-CHANGES IN NEUTRAL LIPID-CONTENT OF PARAMECIUM-PRIMAURELIA AS REVEALED BY NILE RED
Autore:
RAMOINO P; MARGALLO E; NICOLO G;
Indirizzi:
UNIV GENOA,INST ZOOL I-16126 GENOA ITALY NATL INST CANC RES,ANAT & PATHOL CYTOHISTOL SERV I-16132 GENOA ITALY
Titolo Testata:
Journal of lipid research
fascicolo: 6, volume: 37, anno: 1996,
pagine: 1207 - 1212
SICI:
0022-2275(1996)37:6<1207:AINLOP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CULTURE AGE; TETRAURELIA; ATHEROSCLEROSIS; CAUDATUM; STAIN; PROBE; CELLS; CILIA;
Keywords:
CULTURE GROWTH; CLONAL LIFE; LIPID DROPLETS; FOOD INGESTION CAPACITY; FLUORESCENCE MICROSCOPY; CILIATED PROTOZOA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
P. Ramoino et al., "AGE-RELATED-CHANGES IN NEUTRAL LIPID-CONTENT OF PARAMECIUM-PRIMAURELIA AS REVEALED BY NILE RED", Journal of lipid research, 37(6), 1996, pp. 1207-1212

Abstract

Cellular neutral lipid content of Paramecium primaurelia was measuredduring culture and clonal life using nile red (9-diethylamino-5H-benzo[alpha]phenoxazine-5-one), a dye utilized for lipid analysis in both mammalian cells and in ciliated protozoa. Lipid droplets in P. primaurelia are concentrated in the anterior pole of the cell; their number is maximum in early log phase cells and decreases in late log phase cells. The quantitative determination of neutral lipids was obtained measuring the fluorescence from the excitation and emission spectra of 480nm and 540 nm, respectively. Neutral lipid content decreases linearlyduring the log phase of growth while the decline is minimum during the stationary phase. In the late log phase, the amount is 30% of that of the early log-phase cells. Though the cell size declines too, cell area and lipid content decreases are not correlated in the middle log phase, because the maximum lipid reduction is obtained when the cell size is relatively constant. The cellular lipid content also changes during the clonal life. Neutral lipids decrease discontinuously (r = -0.75, P < 0.05) as the fission age increases. No relationship was found between lipid content and food vacuole formation during both culture and clonal life.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 10:14:27