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Titolo:
INFLUENCE OF N-LINKED GLYCANS IN V4-V5 REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GLYCOPROTEIN GP160 ON INDUCTION OF A VIRUS-NEUTRALIZING HUMORAL RESPONSE
Autore:
BOLMSTEDT A; SJOLANDER S; HANSEN JES; AKERBLOM L; HEMMING A; HU SL; MOREIN B; OLOFSSON S;
Indirizzi:
GOTHENBURG UNIV,DEPT CLIN VIROL,GULDHEDSGATAN 10B S-41346 GOTHENBURG SWEDEN GOTHENBURG UNIV,DEPT CLIN VIROL S-41346 GOTHENBURG SWEDEN CTR BIOMED,DEPT VET VIROL UPPSALA SWEDEN HVIDOVRE UNIV HOSP,INFECT DIS LAB HVIDOVRE DENMARK BRISTOL MYERS SQUIBB PHARMACEUT RES INST SEATTLE WA 98121
Titolo Testata:
Journal of acquired immune deficiency syndromes and human retrovirology
fascicolo: 3, volume: 12, anno: 1996,
pagine: 213 - 220
SICI:
1077-9450(1996)12:3<213:IONGIV>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIDS VIRUS; ENVELOPE GLYCOPROTEINS; ANTIGENIC PRESENTATION; MONOCLONAL-ANTIBODIES; GLYCOSYLATION SITES; NUCLEOTIDE-SEQUENCE; RECOMBINANT GP160; DISULFIDE BONDS; GP120; EPITOPES;
Keywords:
IMMUNOGEN; OLIGOSACCHARIDE; HIV-1; GP120; N-LINKAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
A. Bolmstedt et al., "INFLUENCE OF N-LINKED GLYCANS IN V4-V5 REGION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GLYCOPROTEIN GP160 ON INDUCTION OF A VIRUS-NEUTRALIZING HUMORAL RESPONSE", Journal of acquired immune deficiency syndromes and human retrovirology, 12(3), 1996, pp. 213-220

Abstract

One of the functions of N-linked glycans of viral glycoproteins is protecting otherwise accessible neutralization epitopes of the viral envelope from neutralizing antibodies. The aim of the present study was to explore the possibility to obtain a more broadly neutralizing immuneresponse by immunizing guinea pigs with gp160 depleted of three N-linked glycans in the CD4-binding domain by site-directed mutagenesis. Mutant and wild type gp160 were formulated into immunostimulating complexes and injected s.c. into guinea pigs. Both preparations induced highserum antibody response to native gp120 and V3 peptides. Both preparations also induced antibodies that bound equally well to the V3 loop or the CD4-binding region, as determined by a competitive enzyme-linkedimmunosorbent assay (ELISA). The sera from animals, immunized with mutated glycoprotein, did not neutralize nonrelated HIV strains better than did sera from animals, immunized with wild type glycoprotein. Instead, a pattern of preferred homologous neutralization was observed, i.e., sera from animals, immunized with mutant gp160, neutralized mutantvirus better than wild type virus, and vice versa. These data indicated that elimination of the three N-linked glycans from gp160 resulted in an altered local antigenic conformation but did not uncover hidden neutralization epitopes, broadening the immune response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 13:50:43