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Titolo:
AN IMPROVED ISOLATION METHOD FOR MURINE MIGRATORY CUTANEOUS DENDRITICCELLS
Autore:
ORTNER U; INABA K; KOCH F; HEINE M; MIWA M; SCHULER G; ROMANI N;
Indirizzi:
INNSBRUCK UNIV,DEPT DERMATOL,ANICHSTR 35 A-6020 INNSBRUCK AUSTRIA INNSBRUCK UNIV,DEPT DERMATOL A-6020 INNSBRUCK AUSTRIA KYOTO UNIV,DEPT ZOOL KYOTO JAPAN
Titolo Testata:
Journal of immunological methods
fascicolo: 1, volume: 193, anno: 1996,
pagine: 71 - 79
SICI:
0022-1759(1996)193:1<71:AIIMFM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; PROTEIN ANTIGENS; T-CELLS; IDENTIFICATION; GENERATION; ACTIVATION; INVITRO; SYSTEM; SKIN;
Keywords:
DENDRITIC CELL; LANGERHANS CELL; CELL ISOLATION; CUTANEOUS DENDRITIC CELL; (SKIN);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
U. Ortner et al., "AN IMPROVED ISOLATION METHOD FOR MURINE MIGRATORY CUTANEOUS DENDRITICCELLS", Journal of immunological methods, 193(1), 1996, pp. 71-79

Abstract

Dendritic cells are highly specialized accessory cells for the initiation of primary immune responses. They occur as trace populations in non-lymphoid and lymphoid organs. Therefore, the isolation and enrichment of primary dendritic cells is difficult and time-consuming. This applies also to dendritic cells from skin, i.e. epidermal Langerhans cells and dermal dendritic cells. Recently introduced skin organ culturesserve as a convenient source for primary cutaneous dendritic cells. We report here a refinement of such cultures in which cutaneous dendritic cells emigrate spontaneously into the culture medium. Murine ear skin is cultured for a total of 3 days in three sequential 24 h steps. This simple modification doubles or triples the yields of dendritic cells that can be obtained and up to 30 000 dendritic cells can be recovered from one ear half. This represents 50-70% (range 30-80%) of all viable cells. The cells are mature dendritic cells that possess potent Tcell stimulatory function. Compared to the classical methods of preparing epidermal Langerhans cells by trypsinization this technique is easier and quicker; it does not require enzymes such as trypsin, and it yields similar numbers of mature dendritic cells. It should prove useful for further studies of dendritic cells of the skin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:31:54