Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ANTIBODIES AGAINST ADHESION MOLECULES REDUCE APOPTOSIS AFTER TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION IN RAT-BRAIN
Autore:
CHOPP M; LI Y; JIANG N; ZHANG RL; PROSTAK J;
Indirizzi:
HENRY FORD HOSP,DEPT NEUROL,HLTH SCI CTR,2799 W GRAND BLVD DETROIT MI48202 OAKLAND UNIV,DEPT PHYS ROCHESTER MI 00000
Titolo Testata:
Journal of cerebral blood flow and metabolism
fascicolo: 4, volume: 16, anno: 1996,
pagine: 578 - 584
SICI:
0271-678X(1996)16:4<578:AAAMRA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; SYSTEM ISCHEMIC-INJURY; DNA FRAGMENTATION; MONOCLONAL-ANTIBODY; MYOCARDIAL INJURY; SERINE PROTEASES; LYMPHOCYTES-T; TARGET-CELLS; ICAM-1; TISSUE;
Keywords:
APOPTOSIS; ADHESION MOLECULES; ICAM-1; CDLLB; ANTIBODIES; STROKE; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
76
Recensione:
Indirizzi per estratti:
Citazione:
M. Chopp et al., "ANTIBODIES AGAINST ADHESION MOLECULES REDUCE APOPTOSIS AFTER TRANSIENT MIDDLE CEREBRAL-ARTERY OCCLUSION IN RAT-BRAIN", Journal of cerebral blood flow and metabolism, 16(4), 1996, pp. 578-584

Abstract

We tested the hypothesis that treatment of transient focal cerebral ischemia in rat with antibodies directed against adhesion molecules reduces apoptosis. Rats (n = 31) were subjected to 2 h of middle cerebralartery (MCA) occlusion induced by intraluminal insertion of a nylon monofilament into the internal carotid artery, Upon reperfusion, animals were treated with monoclonal antibodies directed against intercellular adhesion molecule (ICAM)-1) (n = 8) or integrin CD11b/CD18 (n = 10), or administered IgG1 as a control (n = 13). At 48 h after ischemia, animals were killed and the brains analyzed for ischemic cell damage, using hematoxylin and eosin (H/E); apoptosis, using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method; and inflammatory cells, using immunohistochemistry withan anti-myeloperoxidase (MPO) antibody. Data revealed a significant reduction in the volume of infarction (p < 0.01) and a decline in the absolute (p < 0.001), and normalized (to the ischemic area, p < 0.05) numbers of apoptotic cells in both animals treated with anti-ICAM-1 andanti-CD11b antibodies compared to control animals. The numbers of immunoreactive MPO cells were also reduced in the treatment groups compared to those in the control group (p < 0.05). These data suggest that treatment with anti-adhesion molecule antibodies selectively reduce apoptosis, and that a contributing factor to the beneficial effect of antibody treatment for reducing ischemic cell damage may be a reduction in numbers of apoptotic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:38:39