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Titolo:
MUSCARINIC AND BETA-ADRENERGIC-RECEPTOR EXPRESSION IN PERIPHERAL LUNGFROM NORMAL AND ASTHMATIC-PATIENTS
Autore:
HADDAD EB; MAK JCW; BELVISI MG; NISHIKAWA M; ROUSELL J; BARNES PJ;
Indirizzi:
NATL HEART & LUNG INST,DEPT THORAC MED,DOVEHOUSE ST LONDON SW3 6LY ENGLAND
Titolo Testata:
American journal of physiology. Lung cellular and molecular physiology
fascicolo: 6, volume: 14, anno: 1996,
pagine: 947 - 953
SICI:
1040-0605(1996)14:6<947:MABEIP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBSTRUCTIVE PULMONARY-DISEASE; AIRWAY SMOOTH-MUSCLE; GUINEA-PIG LUNG; MESSENGER-RNA; SUBTYPES; RESPONSIVENESS; LOCALIZATION; LYMPHOCYTES; INVITRO; BETA-2-ADRENOCEPTORS;
Keywords:
HUMAN LUNG; ASTHMA; RADIOLIGAND BINDING; NORTHERN BLOT ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
E.B. Haddad et al., "MUSCARINIC AND BETA-ADRENERGIC-RECEPTOR EXPRESSION IN PERIPHERAL LUNGFROM NORMAL AND ASTHMATIC-PATIENTS", American journal of physiology. Lung cellular and molecular physiology, 14(6), 1996, pp. 947-953

Abstract

We used human peripheral lung from 8 mildly asthmatic patients and 11normal donors to study the expression of muscarinic and beta-adrenergic receptors in asthma. There was no significant difference in the affinity or the density of muscarinic (labeled with [N-methyl-H-3]scopolamine) and beta(1)- and beta(2)-adrenergic receptors (labeled with [I-125]iodocyanopindolol) in peripheral lung from asthmatics compared withnonasthmatics. Only the muscarinic m(1) receptor mRNA was detected inhuman lung using Northern blot analysis. Additionally, peripheral lung cellular mRNA hybridized to human beta(1) and beta(2) cDNA probes, giving 3.2- and 2.2-kb bands corresponding to beta(1) and beta(2)-adrenergic receptors mRNA, respectively. Densitometric scanning of the autoradiograms suggests that there was no significant difference in the relative abundance of muscarinic m(1) and beta(1)- and beta(2)-adrenergic receptor mRNA in asthmatic compared with nonasthmatic lungs. Functional experiments obtained in trachea suggest that there was an increasein the cholinergic neural response evoked by electrical field stimulation in asthmatic compared with nonasthmatic tissues which was not dueto a reduction in inhibitory noncholinergic nonadrenergic relaxations.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:45:51