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Titolo:
CRYOPRESERVATION OF ISOLATED RAT ISLETS OF LANGERHANS IN THE PRESENCEOF ETHYLENE-GLYCOL OR DIMETHYL-SULFOXIDE - EVALUATION OF TOXICITY ANDTHE DYNAMIC PATTERN OF SUBSEQUENT INSULIN RELEASE IN-VITRO
Autore:
SAKONJU I; TAURA Y; INAYOSHI Y; SUZUKI T; TAKIMOTO K; NAKAICHI M; NAKAMA S;
Indirizzi:
YAMAGUCHI UNIV,FAC AGR,DEPT VET SURG,1677-1 YOSHIDA YAMAGUCHI 735 JAPAN YAMAGUCHI UNIV,FAC AGR,UNITED GRAD SCH VET MED YAMAGUCHI 735 JAPAN
Titolo Testata:
Cryobiology
fascicolo: 3, volume: 33, anno: 1996,
pagine: 354 - 362
SICI:
0011-2240(1996)33:3<354:COIRIO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIMETHYLSULFOXIDE; GLYCEROL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
I. Sakonju et al., "CRYOPRESERVATION OF ISOLATED RAT ISLETS OF LANGERHANS IN THE PRESENCEOF ETHYLENE-GLYCOL OR DIMETHYL-SULFOXIDE - EVALUATION OF TOXICITY ANDTHE DYNAMIC PATTERN OF SUBSEQUENT INSULIN RELEASE IN-VITRO", Cryobiology, 33(3), 1996, pp. 354-362

Abstract

The toxic effect of ethylene glycol (EG) on the pattern of dynamic insulin release from rat pancreatic islets with or without freezing was investigated in comparison with that of dimethyl sulfoxide (Me(2)SO). Sixty islets were perifused (1 ml/min) consecutively with D-glucose (1.67 mM for 30 min followed by 16.7 mM for 60 min and 1.67 mM for 60 min) after exposure to 2.0 M EG or Me(2)SO for 1 h at either 22 or 0 degrees C. During the second period of perifusion, the insulin output from islets exposed to Me(2)SO or EG at 22 degrees C decreased to 53 and 51% of that from nontreated control islets, respectively. On the otherhand, the islets exposed to EG at 0 degrees C exhibited 86% of the control insulin output under the same perifusion conditions, and this appeared to be higher than that of islets exposed to Me(2)SO (60%) at 0 degrees C. Frozen islets, after exposure to 2.0 MEG or Me(2)SO for 1 hat 0 degrees C, responded positively to 16.7 mM D-glucose, and the typical biphasic pattern of insulin secretion was observed. The insulin output from these islets during the second period of perifusion was not comparable to that from unfrozen control islets. In particular, the mean insulin output of EG-cryopreserved islets during the second period accounted for 99% of that from unfrozen control islets. The present findings suggest the possible use of EG as an alternative cryoprotectant to Me(2)SO. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:41:29