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Titolo:
ZINC CHANGES AMPA RECEPTOR PROPERTIES - RESULTS OF BINDING-STUDIES AND PATCH-CLAMP RECORDINGS
Autore:
BRESINK I; EBERT B; PARSONS CG; MUTSCHLER E;
Indirizzi:
MERZ & CO,DEPT PHARMACOL,ECKENHEIMER LANDSTR 100-104 D-60318 FRANKFURT GERMANY MERZ & CO,DEPT PHARMACOL D-60318 FRANKFURT GERMANY ROYAL DANISH SCH PHARM,DEPT ORGAN CHEM,PHARMA BIOTEC DK-2100 COPENHAGEN DENMARK UNIV FRANKFURT,BIOZENTRUM NIEDERURSEL,PHARMAKOL INST NAT WISSENSCH D-60053 FRANKFURT GERMANY
Titolo Testata:
Neuropharmacology
fascicolo: 4, volume: 35, anno: 1996,
pagine: 503 - 509
SICI:
0028-3908(1996)35:4<503:ZCARP->2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYL-D-ASPARTATE; MOUSE HIPPOCAMPAL-NEURONS; AMINO-ACID RECEPTORS; RAT-BRAIN; SINGLE RECEPTOR; KAINATE; DESENSITIZATION; ACTIVATION; MEMBRANES; NEUROTOXICITY;
Keywords:
ZINC; [H-3] AMPA; CORTEX; SUPERIOR COLLICULUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
I. Bresink et al., "ZINC CHANGES AMPA RECEPTOR PROPERTIES - RESULTS OF BINDING-STUDIES AND PATCH-CLAMP RECORDINGS", Neuropharmacology, 35(4), 1996, pp. 503-509

Abstract

The influence of zinc ions on lpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors was investigated using binding studies with [H-3]AMPA to rat cortical membranes and patch clamp recordingsfrom cultured superior colliculus neurones. In Tris-HCl buffer, zinc (1-10 mM) significantly increased the specific binding of [H-3]AMPA whereas this increase was negligible in the presence of CaCl2 (2.5 mM) and KSCN (100 mM). This effect was associated with a dramatic increase in B-max but a decrease in both agonist and antagonist affinity. Association and dissociation experiments showed that equilibrium [H-3]AMPA binding is reached with faster kinetics in the presence of zinc. At low concentrations (0.3 mM) zinc also concentration-dependently potentiated both peak and plateau components of whole cell current responses to AMPA (100 mu M) This effect was accompanied by a reduction of the degree, and slowing of the rate, of AMPA receptor desensitisation. In contrast, higher concentrations of zinc (1-3.0 mM) inhibited AMPA responses to some degree, but slowed desensitisation further. This ability of zinc to change AMPA receptor properties may be relevant to neurotoxicity associated with AMPA receptor activation. (C) 1996 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:05:34