Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DETECTION AND QUANTIFICATION OF DEGRADATIVE GENES IN SOILS CONTAMINATED BY TOLUENE
Autore:
HALLIERSOULIER S; DUCROCQ V; MAZURE N; TRUFFAUT N;
Indirizzi:
UNIV TECHNOL COMPIEGNE,LAB GENET MICROBIENNE,BP 649 F-60206 COMPIEGNEFRANCE UNIV TECHNOL COMPIEGNE,LAB GENET MICROBIENNE F-60206 COMPIEGNE FRANCE
Titolo Testata:
FEMS microbiology, ecology
fascicolo: 2, volume: 20, anno: 1996,
pagine: 121 - 133
SICI:
0168-6496(1996)20:2<121:DAQODG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; DIRECT DNA EXTRACTION; ESCHERICHIA-COLI; ENVIRONMENTAL MICROBIOLOGY; PSEUDOMONAS; BACTERIA; SEDIMENTS; PERSISTENCE; EXPRESSION; PLASMIDS;
Keywords:
TOLUENE BIODEGRADATION; XYLE GENE; SOIL MICROCOSM; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
S. Halliersoulier et al., "DETECTION AND QUANTIFICATION OF DEGRADATIVE GENES IN SOILS CONTAMINATED BY TOLUENE", FEMS microbiology, ecology, 20(2), 1996, pp. 121-133

Abstract

A method based on the polymerase chain reaction (PCR) was developed for a rapid and specific detection of toluene degradative genes in soilThe xylE gene coding for catechol 2,3-dioxygenase was chosen as a target gene. The detection threshold was evaluated in microcosms using a sterilized standard soil inoculated with various amounts of a degradative strain of Pseudomonas putida (mX). The extracted DNA was used as atemplate to amplify the xylE gene. PCR followed by hybridization withan internal probe allowed us to detect 10(2) bacteria per g of soil. In polluted soils, quantification of target DNA by competitive PCR wascompared with enumeration of degradative microflora. This molecular method appeared;to be rapid, sensitive and more suitable than the microbiological approach to estimate the biodegradative potential of a polluted soil.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:35:15