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Titolo:
A TRI-HYBRID SYSTEM FOR THE ANALYSIS AND DETECTION OF RNA-PROTEIN INTERACTIONS
Autore:
PUTZ U; SKEHEL P; KUHL D;
Indirizzi:
UNIV HAMBURG,CTR MOL NEUROBIOL ZMNH,MARTINISTR 52 D-20246 HAMBURG GERMANY UNIV HAMBURG,CTR MOL NEUROBIOL ZMNH D-20246 HAMBURG GERMANY NATL INST MED RES,DIV NEUROPHYSIOL LONDON NW7 1AA ENGLAND
Titolo Testata:
Nucleic acids research
fascicolo: 23, volume: 24, anno: 1996,
pagine: 4838 - 4840
SICI:
0305-1048(1996)24:23<4838:ATSFTA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
REV TRANS-ACTIVATOR; MESSENGER-RNA; EXPRESSION; BINDING; YEAST; GENE; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
U. Putz et al., "A TRI-HYBRID SYSTEM FOR THE ANALYSIS AND DETECTION OF RNA-PROTEIN INTERACTIONS", Nucleic acids research, 24(23), 1996, pp. 4838-4840

Abstract

A modification of the two-hybrid system is described for the in vivo reconstruction of specific RNA-protein interactions, In this tri-hybrid system, the DNA binding and transcription activation domains of the yeast transcriptional activator GAL4 are brought together via the interaction of recombinant fusion proteins with a recombinant RNA, The method provides a system for studying RNA-protein interactions with the genetic advantages of the two-hybrid system, It may be used to detect specific RNA-binding proteins or target RNAs from a library of cDNAs, or to analyse the structural specificity of identified RNA-protein interactions.

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Documento generato il 11/07/20 alle ore 20:44:26