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Titolo:
INVASION OF TISSUE-CULTURE CELLS BY DIARRHOEAGENIC STRAINS OF ESCHERICHIA-COLI WHICH LACK THE ENTEROINVASIVE INV GENE
Autore:
GEYID A; FLETCHER J; GASHE BA; LJUNGH A;
Indirizzi:
LUND UNIV,DEPT MED MICROBIOL,SOLVEGATAN 23 S-22362 LUND SWEDEN LUND UNIV,DEPT MED MICROBIOL S-22362 LUND SWEDEN NATL RES INST HLTH,DEPT BACTERIOL ADDIS ABABA ETHIOPIA UNIV LIVERPOOL,DEPT MED MICROBIOL LIVERPOOL L69 3BX MERSEYSIDE ENGLAND UNIV ADDIS ABABA,DEPT BIOL ADDIS ABABA ETHIOPIA
Titolo Testata:
FEMS immunology and medical microbiology
fascicolo: 1, volume: 14, anno: 1996,
pagine: 15 - 24
SICI:
0928-8244(1996)14:1<15:IOTCBD>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CACO-2 EPITHELIAL-CELLS; BUNDLE-FORMING PILUS; HEP-2 CELLS; SALMONELLA-TYPHIMURIUM; YERSINIA-PSEUDOTUBERCULOSIS; BACTERIAL ATTACHMENT; EFFACING LESIONS; ADHERENCE FACTOR; MAMMALIAN-CELLS; GROWTH-PHASE;
Keywords:
ESCHERICHIA COLI; ADHESION; INVASION; TISSUE CULTURE CELL; IRON; GROWTH CONDITION; GLUCOSE; MANNOSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
A. Geyid et al., "INVASION OF TISSUE-CULTURE CELLS BY DIARRHOEAGENIC STRAINS OF ESCHERICHIA-COLI WHICH LACK THE ENTEROINVASIVE INV GENE", FEMS immunology and medical microbiology, 14(1), 1996, pp. 15-24

Abstract

Invasive Escherichia coli strains of certain serotypes invade by the same mechanism as the Shigella sp. It has been proposed that invasion of epithelial cells by EPEC strains may also occur; this is a previously overlooked property. In the present study E. coli strains isolated from patients with diarrhoea or ulcerative colitis, lacking the inv plasmid mediating classical invasion, but hybridizing with probes for different adhesins, were analyzed for their ability to invade HeLa and Caco-2 cells, The majority of strains invaded Caco-2 cells to a higher extent than HeLa cells, Adhesion to Caco-2 cells was a prerequisite for subsequent invasion of the cells but EAF, ene, EAgg and other known virulence factors were not sufficient to mediate invasion. In 8/9 E. coli strains invasion was enhanced after growth under iron restriction. Growth during anaerobic conditions did not influence subsequent invasion by E. coli strains whereas 6/9 strains had their invasive ability significantly decreased after growth in the presence of 1% glucose. The invasive process was inhibited by mannose but not by lactose, fucoseor galactose. Our data indicate that strains of E. coli may invade Caco-2 cells by novel mechanisms which require adhesion to the cells butwhich differ from those of Salmonella sp., Yersinia sp., Shigella sp.and classical enteroinvasive E. coli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 00:50:34