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Titolo:
BORON CONTROLS SUSPENSOR DEVELOPMENT IN EMBRYOGENIC CULTURES OF LARIX-DECIDUA
Autore:
BEHRENDT U; ZOGLAUER K;
Indirizzi:
HUMBOLDT UNIV BERLIN,INST BIOL,INVALIDENSTR 43 D-10115 BERLIN GERMANY
Titolo Testata:
Physiologia Plantarum
fascicolo: 2, volume: 97, anno: 1996,
pagine: 321 - 326
SICI:
0031-9317(1996)97:2<321:BCSDIE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC EMBRYOGENESIS; CELL-CULTURES; DAUCUS-CAROTA; WHITE SPRUCE; PICEA-GLAUCA; REGENERATION; NUTRITION; INITIATION; CONIFERS; CALLUS;
Keywords:
BORON; LARCH; LARIX DECIDUA; PROTOPLASTS; SOMATIC EMBRYOGENESIS; SUSPENSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
U. Behrendt e K. Zoglauer, "BORON CONTROLS SUSPENSOR DEVELOPMENT IN EMBRYOGENIC CULTURES OF LARIX-DECIDUA", Physiologia Plantarum, 97(2), 1996, pp. 321-326

Abstract

Suspensor formation in somatic embryos of Larix decidua Mill. was studied in relation to its dependence on nutritional factors of the culture medium. Protoplasts exhibiting embryogenic competence were preparedfrom an embryogenic suspension culture, immobilized in Ca-alginate and cultured in liquid media. Developmental patterns showed qualitative differences in the two basic media used. In one medium, containing 100mu M boric acid, compact colonies developed, most of which displayed formation of polar suspensors after 3 to 4 weeks. In contrast, suspensor development never occurred in the other medium, containing 10 mu M berate, yet colonies of loosely connected cytoplasmically dense cells were regenerated without loss of embryogenic competence. Detailed examination of the constituents of the two media led to the conclusion that suspensor development is dependent on boron concentration: below 35 mu M boron, suspensor formation is blocked completely, whereas with anincrease up to 1 mM boron there is a progressively faster developmentof suspensor-bearing embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 07:39:33