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Titolo:
EFFECT OF GINSENOSIDE RB-1 ON RAT-LIVER PHOSPHOPROTEINS INDUCED BY CARBON-TETRACHLORIDE
Autore:
PARK HJ; PARK KM; RHEE MH; SONG YB; CHOI KJ; LEE JH; KIM SC; PARK KH;
Indirizzi:
KOREA GINSENG & TOBACCO RES INST,DEPT BIOCHEM PHARMACOL,YUSUNG KU TAEJON 305345 SOUTH KOREA
Titolo Testata:
Biological & pharmaceutical bulletin
fascicolo: 6, volume: 19, anno: 1996,
pagine: 834 - 838
SICI:
0918-6158(1996)19:6<834:EOGROR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN KINASE-C; GLYCOGEN-SYNTHASE; PLASMA-MEMBRANES; CALCIUM-PUMP; (CA2+-MG2+)-ATPASE; PHOSPHORYLATION; INHIBITORS; CALMODULIN; ACTIVATION; MECHANISM;
Keywords:
GINSENOSIDE RB-1; CARBON TETRACHLORIDE; PROTEIN PHOSPHORYLATION; RAT LIVER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
H.J. Park et al., "EFFECT OF GINSENOSIDE RB-1 ON RAT-LIVER PHOSPHOPROTEINS INDUCED BY CARBON-TETRACHLORIDE", Biological & pharmaceutical bulletin, 19(6), 1996, pp. 834-838

Abstract

We investigated the effects of ginsenoside Rb-1 (G-Rb-1), a major saponin from Panax ginseng C. A. MEYER, on rat liver protein phosphorylation after intraperitoneal administration of CCl4 alone or together with G-Rb-1. We found that 118, 63, and 34 kDa proteins were prominently phosphorylated in liver homogenates prepared from CCl4-administered rats, while these protein-phosphorylations were inhibited in the homogenate prepared from the G-Rb-1 plus CCl4-administration group, When inhibitors of protein kinases were exogenously added to the homogenates from either the CCl4-administered group or the G-Rb-1 plus CCl4-administered group, their phosphorylations were inhibited much more by W-7, aninhibitor of Ca2+/calmodulin-dependent protein kinase (CaM-PK), than by H-7, an inhibitor of protein kinase C (C-kinase), Interestingly, only 34 kDa was phosphorylated in homogenates prepared from the corn oil-, G-Rb-1-, and G-Rb-1 plus CCl4-administered groups by the exogenous addition of sodium fluoride (NaF), an inhibitor of glycogen synthase, Additionally, G-Rb-1 inhibited the Ca2+-accumulation induced by CCl4 both in liver homogenates and microsomes, The above results imply that G-Rb-1 inhibits the CCl4-induced protein phosphorylations by modulating CaM-PK rather than C-kinase, and that 34 kDa protein may play a different biological role in cellular environment from 118 and 63 kDa proteins, Therefore, a study in which G-Rb-1 is employed as a modulator ofcritical CCl4-induced phenomena ranging from the disturbance of Ca2+ concentration to protein phosphorylation may be successfully applicable to investigate the diverse physiological functions of liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 19:51:42