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Titolo:
ACUTE STIMULATION OF INTESTINAL-CELL CALCIUM INFLUX INDUCED BY 17-BETA-ESTRADIOL VIA THE CAMP MESSENGER SYSTEM
Autore:
PICOTTO G; MASSHEIMER V; BOLAND R;
Indirizzi:
UNIV NACL SUR,DEPT BIOL & BIOQUIM,SAN JUAN 670 RA-8000 BAHIA BLANCA ARGENTINA UNIV NACL SUR,DEPT BIOL & BIOQUIM RA-8000 BAHIA BLANCA ARGENTINA
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 2, volume: 119, anno: 1996,
pagine: 129 - 134
SICI:
0303-7207(1996)119:2<129:ASOICI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-D METABOLITES; ESTROGEN-RECEPTOR; BONE LOSS; ABSORPTION; ADENOSINE; AGE; TRANSCALTACHIA; MONOPHOSPHATE; MODULATION; TRANSPORT;
Keywords:
ENTEROCYTES; INTESTINE; 17-BETA-ESTRADIOL; STEROID HORMONES; CALCIUM CHANNELS; CAMP SIGNALING PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
G. Picotto et al., "ACUTE STIMULATION OF INTESTINAL-CELL CALCIUM INFLUX INDUCED BY 17-BETA-ESTRADIOL VIA THE CAMP MESSENGER SYSTEM", Molecular and cellular endocrinology, 119(2), 1996, pp. 129-134

Abstract

Recent studies have provided evidence for nuclear estrogen receptor-mediated calcium transport in intestinal mucosal cells. The possibilitythat, in addition, estrogens directly stimulate intestinal Ca2+ flu?res through second-messenger pathways was investigated. Exposure of enterocytes isolated from female rat duodenum to low physiological levelsof 17 beta-estradiol (10(-11), 10(-10) and 10(-8) M) rapidly (1-10 min) increased (50-170%) cell Ca-45(2+) influx. 17 alpha-Estradiol, dihydrotestosterone and progesterone were devoid of activity, suggesting specificity of the estrogen effect. Maximum responses induced by 17 beta-estradiol (5 min at 10(-10) M) could be abolished to a great extent (84%) by pretreating the cells with verapamil (10 mu M) and nitrendipine (1 mu M), involving the activation of voltage-dependent Ca2+ channels in the fast increase of rat duodenal calcium uptake by the hormone. Evidence was obtained indicating that the acute estrogen stimulation of enterocyte Ca2+ influx is mediated by the cyclic AMP/PKA pathway. 17 beta-Estradiol rapidly increased cAMP content of rat duodenal cells in parallel to the changes in Ca2+ uptake. In addition, forskolin, dibutyryl cAMP and Sp-cAMPS mimicked and Rp-cAMPS suppressed the prompt 17 beta-estradiol-induced stimulation of Ca2+ influx. These results areconsistent with a direct action of estrogens in the enterocyte, presumably a non-genomic one, initiated on the cell surface and resulting in rapid activation of the cAMP pathway and Ca2+ channels, which may berelevant for regulation of intestinal calcium transport.

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Documento generato il 16/07/20 alle ore 06:14:19