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Titolo:
CANNABINOIDS INHIBIT AGONIST-STIMULATED FORMATION OF INOSITOL PHOSPHATES IN RAT HIPPOCAMPAL CULTURES
Autore:
NAH SY; SAYA D; VOGEL Z;
Indirizzi:
WEIZMANN INST SCI,DEPT NEUROBIOL,POB 26 IL-76100 REHOVOT ISRAEL WEIZMANN INST SCI,DEPT NEUROBIOL,POB 26 IL-76100 REHOVOT ISRAEL
Titolo Testata:
European journal of pharmacology. Molecular pharmacology section
fascicolo: 1, volume: 246, anno: 1993,
pagine: 19 - 24
SICI:
0922-4106(1993)246:1<19:CIAFOI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUROBLASTOMA-CELLS; ADENYLATE-CYCLASE; BRAIN; METABOLISM; DELTA-9-TETRAHYDROCANNABINOL; RECEPTORS; SLICES;
Keywords:
CANNABINOID; HIPPOCAMPUS; INOSITOL PHOSPHATES; PHOSPHATIDYLINOSITOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
S.Y. Nah et al., "CANNABINOIDS INHIBIT AGONIST-STIMULATED FORMATION OF INOSITOL PHOSPHATES IN RAT HIPPOCAMPAL CULTURES", European journal of pharmacology. Molecular pharmacology section, 246(1), 1993, pp. 19-24

Abstract

The effect of cannabinoids on phosphoinositide metabolism stimulated by activation of muscarinic receptors, alpha1-adrenoceptors or glutamate receptors was examined in rat hippocampal cultures. Carbachol stimulated phosphoinositide turnover by 5.5-fold over basal level, whereas glutamate and norepinephrine stimulated phosphoinositide turnover by 2-fold. Addition of cannabinoids, such as DELTA8-tetrahydrocannabinol, DELTA9-tetrahydrocannabinol or the psychoinactive cannabidiol inhibited formation of inositol phosphates evoked by carbachol, glutamate or norepinephrine by 55-90%. The cannabinoids alone only slightly inhibited the basal unstimulated formation of inositol phosphates. The inhibitory effect of the cannabinoids was dose-dependent and was achieved within the range of pharmacologically relevant concentrations. IC50 values for DELTA8-tetrahydrocannabinol, DELTA9-tetrahydrocannabinol and cannabidiol were 9.6 +/- 1.0, 9.7 +/- 0.3 and 7.9 +/- 0.4 muM, respectively. Pretreatment with pertussis toxin (100 ng/ml, 18 h) did not affectthe carbachol-induced stimulation of phosphoinositide turnover or itsinhibition by the cannabinoids. This suggests that the inhibition by the cannabinoids of the stimulated formation of inositol phosphates isnot mediated through a pertussis toxin-sensitive GTP-binding protein nor through the known effect of the cannabinoids on adenylate cyclase inhibition.

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Documento generato il 27/09/20 alle ore 01:03:59