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Titolo:
TERTIARY STRUCTURE COMPARISON OF BROMELAIN INHIBITOR-VI FROM PINEAPPLE STEM WITH BOVINE PANCREATIC TRYPSIN-INHIBITOR
Autore:
HATANO K; TANOKURA M; TAKAHASHI K;
Indirizzi:
UNIV TOKYO,FAC SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,BIOTECHNOL RES CTR,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
Proceedings of the Japan Academy. Series B Physical and biological sciences
fascicolo: 5, volume: 72, anno: 1996,
pagine: 104 - 107
SICI:
0386-2208(1996)72:5<104:TSCOBI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYSTEINE PROTEINASES;
Keywords:
BROMELAIN INHIBITOR VI; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; STRUCTURAL COMPARISON; SOLVENT ACCESSIBILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
K. Hatano et al., "TERTIARY STRUCTURE COMPARISON OF BROMELAIN INHIBITOR-VI FROM PINEAPPLE STEM WITH BOVINE PANCREATIC TRYPSIN-INHIBITOR", Proceedings of the Japan Academy. Series B Physical and biological sciences, 72(5), 1996, pp. 104-107

Abstract

Bromelain inhibitor VI from pineapple stem (BI-VI) is a unique double-chain cysteine proteinase inhibitor composed of an 11-residue light chain and a 41-residue heavy chain linked by disulfide bonds. The positions of six of the ten half-cystines in the primary structure of BI-VIresemble those of the six half-cystines in bovine pancreatic trypsin inhibitor (BPTI), a serine proteinase inhibitor which apparently shares some properties with BI-VI. These facts suggested that they might resemble in their three-dimensional structures. To clarify this point, the solution structure of BI-VI was elucidated using nuclear magnetic resonance spectroscopy and simulated annealing-based calculations. Thus, the inhibitor was shown to be composed of two distinct domains, eachconstructed by a three-stranded antiparallel beta-sheet. Comparison with BPTI revealed conclusively that BI-VI is distinctly different in three-dimensional structure from BPTI.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:34:51