Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LOCALIZATION AND EXPRESSION OF BETA-ADRENOCEPTOR SUBTYPE MESSENGER-RNAS IN HUMAN LUNG
Autore:
MAK JCW; NISHIKAWA M; HADDAD EB; KWON OJ; HIRST SJ; TWORT CHC; BARNES PJ;
Indirizzi:
UMDS,ST THOMAS HOSP,NATL HEART & LUNG INST,DEPT THORAC MED,SMOOTH MUSCLE RES GRP,DIV MED LONDON SW3 ENGLAND UMDS,ST THOMAS HOSP,NATL HEART & LUNG INST,DEPT THORAC MED,SMOOTH MUSCLE RES GRP,DIV MED LONDON SW3 ENGLAND UMDS,ST THOMAS HOSP,RESP RES LABS,SMOOTH MUSCLE RES GRP,DIV MED LONDON SW3 ENGLAND
Titolo Testata:
European journal of pharmacology
fascicolo: 1-3, volume: 302, anno: 1996,
pagine: 215 - 221
SICI:
0014-2999(1996)302:1-3<215:LAEOBS>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRONCHIAL SMOOTH-MUSCLE; RECEPTOR MESSENGER-RNA; BETA-3-ADRENERGIC RECEPTOR; ADRENERGIC-RECEPTOR; CLONING; CELLS; ADIPOSE; CDNA;
Keywords:
BETA-ADRENOCEPTOR; MESSENGER-RNA; HYBRIDIZATION, IN SITU; NORTHERN BLOT ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
J.C.W. Mak et al., "LOCALIZATION AND EXPRESSION OF BETA-ADRENOCEPTOR SUBTYPE MESSENGER-RNAS IN HUMAN LUNG", European journal of pharmacology, 302(1-3), 1996, pp. 215-221

Abstract

The cellular localisation and distribution of mRNAs encoding beta-adrenoceptor subtypes in human lung were studied by in situ hybridisationand Northern blot analysis. The 851-bp SmaI/PvuII fragment of human beta(1)-adrenoceptor cDNA, the 439-bp SmaI fragment of human beta(2)-adrenoceptor cDNA and the 975-bp SmaI fragment of human beta(3)-adrenoceptor cDNA bound to single mRNA species of approximately 3.2 kb, 2.2 kband 2.3 kb in size, respectively. Human lung and heart and rabbit lung expressed both beta(1)- and beta(2)-adrenoceptor mRNAs with no detectable level of beta(3)-adrenoceptor mRNA, while rabbit perirenal adipose tissue expressed beta(1)-, beta(2)- and beta(3)-adrenoceptor mRNAs. Cultured human airway epithelial cells and airway smooth muscle cellsexpressed only beta(2)-adrenoceptor mRNA. In situ hybridisation in human lung, using S-35-labelled antisense RNA probes revealed a high level of expression of beta(1)- and beta(2)-adrenoceptor mRNAs in the pulmonary blood vessels, high level of expression of beta(2)-adrenoceptormRNA in the alveolar walls with less expression of beta(1)-adrenoceptor mRNA. There was a moderate expression of beta(2)-adrenoceptor but not beta(1)-adrenoceptor mRNA in airway epithelium and smooth muscle ofperipheral airways and no delectable beta(3)-adrenoceptor mRNA in anylung structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 12:43:04