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Titolo:
PURIFICATION AND PARTIAL CHARACTERIZATION OF A LUTEIN-BINDING PROTEINFROM THE MIDGUT OF THE SILKWORM BOMBYX-MORI
Autore:
JOUNI ZE; WELLS MA;
Indirizzi:
UNIV ARIZONA,DEPT BIOCHEM,POB 210066,BIOL SCI W TUCSON AZ 85721
Titolo Testata:
The Journal of biological chemistry
fascicolo: 25, volume: 271, anno: 1996,
pagine: 14722 - 14726
SICI:
0021-9258(1996)271:25<14722:PAPCOA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESONANCE RAMAN; CANTHAXANTHIN-LIPOVITELLIN; BLUE CAROTENOPROTEIN; CIRCULAR-DICHROISM; CRUSTACEA; ANOSTRACA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
Z.E. Jouni e M.A. Wells, "PURIFICATION AND PARTIAL CHARACTERIZATION OF A LUTEIN-BINDING PROTEINFROM THE MIDGUT OF THE SILKWORM BOMBYX-MORI", The Journal of biological chemistry, 271(25), 1996, pp. 14722-14726

Abstract

A lutein-binding protein was purified from fifth instar larval midgutof Bombyx mori by a combination of ammonium sulfate fractionation andthree chromatographic procedures, gel filtration, chromatofocusing, and anion exchange chromatography. The protein has a pi of 5.4 and an apparent molecular mass of 35,000 Da, as determined by a linear gradient SDS-polyacrylamide gel electrophoresis. The lutein-protein complex is water-soluble and more stable than the carotenoid or protein alone. The carotenoid moiety was identified by thin layer chromatography, light absorption spectroscopy, and high performance liquid chromatographyas all-trans-lutein. Lutein is specifically and stoichiometrically bound to the protein, with a ratio of 3 mol of lutein per mol of protein. Binding of lutein (absorption maxi mum in hexane at 454 nm) to the apoprotein results in a marked red spectral shift of about 38 nm, giving rise to absorption maxima at 432, 462, and 492 nm in 20 mM Tris-HCl,pH 7.0. The lutein-protein complex is characterized by fine spectral structure indicating that lutein is in a relatively rigid environment. This protein is distributed in equal amounts throughout the midgut and in all developmental stages of the larval B. mori.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 05:21:32