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Titolo:
MOLECULAR-WEIGHT DETERMINATION OF PLASMID DNA USING ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
Autore:
CHENG XH; CAMP DG; WU QY; BAKHTIAR R; SPRINGER DL; MORRIS BJ; BRUCE JE; ANDERSON GA; EDMONDS CG; SMITH RD;
Indirizzi:
BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM MOL SCI LAB,POB 999 RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM MOL SCI LAB RICHLAND WA 99352 EASTERN OREGON STATE COLL,DEPT CHEM LA GRANDE OR 97850 BATTELLE MEM INST,PACIFIC NW LABS,DEPT BIOL & CHEM RICHLAND WA 99352
Titolo Testata:
Nucleic acids research
fascicolo: 11, volume: 24, anno: 1996,
pagine: 2183 - 2189
SICI:
0305-1048(1996)24:11<2183:MDOPDU>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLY CHARGED IONS; HUMAN GENOME; BIOMOLECULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
X.H. Cheng et al., "MOLECULAR-WEIGHT DETERMINATION OF PLASMID DNA USING ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY", Nucleic acids research, 24(11), 1996, pp. 2183-2189

Abstract

lonization and molecular weight (MW) determination of megadalton sizeplasmid DNA has been achieved using electrospray ionization (ESI) with Fourier transform ion cyclotron resonance (FTICR) mass spectrometry,DNA molecules were shown to remain intact through electrospray ionization by collection on a specially prepared surface, followed by agarose gel electrophoresis. Individual highly charged ions of plasmid DNA produced by ESI were trapped in an FTICR cell for up to several hours and reacted with acetic acid to induce charge state shifts, Measurements of mass-to-charge ratios for these multiple peaks arising from charge state shifting give MW measurements of individual ions with an average accuracy of 0.2%, The MW distribution was obtained by measurements for a number of individual ions from the same sample [plasmid DNA: pGEM-5S MW(cal) = 1.946 MDa], yielding a MW(obs) of 1.95 +/- 0.07 MDa forions clustered in the vicinity of the expected MW.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/08/20 alle ore 20:26:18