Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ISOLATION AND CHARACTERIZATION OF A CHINESE-HAMSTER OVARY MUTANT-CELLLINE WITH ALTERED SENSITIVITY TO VACCINIA VIRUS KILLING
Autore:
BAIR CH; CHUNG CS; VASILEVSKAYA IA; CHANG W;
Indirizzi:
ACAD SINICA,INST MOLEC BIOL NANKANG TAIWAN ACAD SINICA,INST MOLEC BIOL NANKANG TAIWAN NATL DEF MED CTR,GRAD INST LIFE SCI TAIPEI 115 TAIWAN
Titolo Testata:
Journal of virology
fascicolo: 7, volume: 70, anno: 1996,
pagine: 4655 - 4666
SICI:
0022-538X(1996)70:7<4655:IACOAC>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT RNA-POLYMERASE; RETROVIRUS PROMOTER TRAP; PSEUDOMONAS EXOTOXIN-A; CHLORAMPHENICOL ACETYLTRANSFERASE; PHENOTYPIC CHARACTERIZATION; ECTROMELIA VIRUS; GENE-EXPRESSION; DNA-REPLICATION; STEM-CELLS; INFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
C.H. Bair et al., "ISOLATION AND CHARACTERIZATION OF A CHINESE-HAMSTER OVARY MUTANT-CELLLINE WITH ALTERED SENSITIVITY TO VACCINIA VIRUS KILLING", Journal of virology, 70(7), 1996, pp. 4655-4666

Abstract

The Chinese hamster ovary (CHO) cell line is nonpermissive for vaccinia virus, and translation of viral intermediate genes was reported to be blocked (A, Ramsey-Ewing and B. Moss, Virology 206:984-993, 1995), However, cells are readily killed by vaccinia virus, A vaccinia virus-resistant CHO mutant, VV5-4, was isolated by retroviral insertional mutagenesis. Parental CHO cells, upon infection with vaccinia virus, diewithin 2 to 3 days, whereas VV5-4 cells preferentially survive this cytotoxic effect, The survival phenotype of VV5-4 is partial and in inverse correlation with the multiplicity of infection used, In addition,viral infection fails to shut off host protein synthesis in VV5-4, VV5-4 was used to study the relationship of progression of the virus life cycle and cell fate, We found that in parental CHO cells, vaccinia virus proceeds through expression of viral early genes, uncoating, viral DNA replication, and expression of intermediate and late promoters, In contrast, we detect only expression of early genes and uncoating inVV5-4 cells, whereas viral DNA replication appears to be blocked, Consistent with the cascade regulation model of viral gene expression, wedetect little intermediate- and late-gene expression in VV5-4 cells, Since vaccinia virus is known to be cytolytic, isolation of this mutant therefore demonstrates a new mode of the cellular microenvironment that affects progression of the virus life cycle, resulting in a different cell fate. This process appears to be mediated by a general mechanism, since VV5-4 is also resistant to Shope fibroma virus and myxoma virus killing, On the other hand, VV5-4 remains sensitive to cowpox virus killing. To examine the mechanism of VV5-4 survival, we investigated whether apoptosis is involved, DNA laddering and staining of apoptotic nuclei with Hoechst 33258 were observed in both CHO and VV5-4 cellsinfected with vaccinia virus, We concluded that the cellular pathway,which blocks viral DNA replication and allows VV5-4 to survive, is independent of apoptosis, This mutant also provides evidence that an inductive signal for apoptosis upon vaccinia virus infection occurs priorto viral DNA replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:32:51